传感技术学报2017，Vol.30Issue(2)：218-223,6.DOI:10.3969/j.issn.1004-1699.2017.02.009
基于微环形阵列电极的细胞分离富集芯片的设计与实验研究
Design and Experimental Study of Cells Isolation and EnrichmentChip-Based on Micro Annular Array Electrode
摘要
Abstract
According to the theory of dielectrophoresis,a kind of cells isolation and enrichment chip of micro annular array was designed and modeled.COMSOL software was used to discover the position of maximum and minimum strength of electric field via analysing the distribution of electric field and the direction of dielectrophoresis force.The chip was fabricated based on ITO glass and PDMS.The cross frequency of yeast cells was obtained after conducting a dielectrophoretic enrichment experiment of yeast cells.Isolation and enrichment of yeast cells and polystyrene particles were realized through carrying out an isolation and enrichment experiment with yeast cells and polystyrene particles.The results showed that yeast cells in solution conductivity of 60 μs/cm did negative DEP response and enriched in the ring inside under a voltage of 8 V p-p and the frequency of 1 kHz to 45 kHz.The cross frequency of yeast cells was determined to be 45 kHz.Yeast cells did positive DEP response and enriched along the ring edge in the range of 45 kHz to 10 MHz.The polystyrene particles did negative DEP response and enriched in the ring inside under a frequency of 1.5 MHz.The multiple of enrichment reached about 11.66.关键词
生物芯片/细胞分离富集/介电泳/微环形电极/COMSOLKey words
biological chip/cells isolation and enrichment/dielectrophoresis/micro annular electrode/COMSOL分类
信息技术与安全科学引用本文复制引用
樊磊,曾一笑,吴菲,谭秋林,孙东..基于微环形阵列电极的细胞分离富集芯片的设计与实验研究[J].传感技术学报,2017,30(2):218-223,6.基金项目
山西省百人计划 （）
西省基础研究项目(2014011021-3) （2014011021-3）
