重庆医学2016，Vol.45Issue(33)：4633-4635,3.DOI:10.3969/j.issn.1671-8348.2016.33.008
Apelin对葡萄糖的毒性作用及对胰岛细胞PDX-1表达的影响
Effects of Apelin on glucose toxicity and islet cells PDX-1 expression
摘要
Abstract
Objective To study the effects of Apelin on glucose toxicity and islet cells PDX-1 protein expression.Methods The islet β cell line NIT-1 cells were incubated in the medium containing different glucose concentrations(normal glucose concentration group 5.6 mmol/L,high glucose concentration group 16.7 mmol/L,extremely high glucose concentration group 33.3 mmol/L) and +/-Apelin-36 respectively for 3 d.Then the basic insulin secretion amount of islet cells and their secretion amount after glucose stimulation were detected.The intracellular insulin content and the PDX-1 protein and mRNA expression were detected.Results Compared with the normal glucose group,the basic insulin secretion,secretion after stimulation and intracellular insulin in the high glucose group and extremely high glucose group were significantly decreased and PDX-1 protein expression was declined(P＜ 0.05);compared with non-adding Apelin group,the basic insulin secretion,secretion after stimulation and intracellular insulin in the adding Apelin high glucose group and extremely high glucose group were significantly decreased and PDX-1 protein expression was decreased(P＜0.05);the insulin level in islet cells of 6 groups was positively correlated with PDX-1 protein expression and had no correlation with PDX-1 mRNA expression.Conclusion Apelin may participate in the glucose toxic effect by decreasing PDX-1 protein expression,causes the decrease of insulin secretion,thus plays a role in the pathogenesis of diabetes.关键词
Apelin/胰十二指肠同源盒蛋白-1/葡萄糖毒性Key words
Apelin/pancreatic duodenal homeobox-1/glucose toxicity分类
医药卫生引用本文复制引用
黄炫赓,黎英荣,郑晓菲,潘海林,苏宏业,夏宁,肖常青..Apelin对葡萄糖的毒性作用及对胰岛细胞PDX-1表达的影响[J].重庆医学,2016,45(33):4633-4635,3.基金项目
广西壮族自治区卫生厅重点课题(桂卫重2010024). （桂卫重2010024）
