重庆医学2017，Vol.46Issue(10)：1300-1303,1307,5.DOI:10.3969/j.issn.1671-8348.2017.10.002
TERT基因转染BMSC血管性痴呆大鼠记忆功能及海马CA1区突触可塑性的影响
Effect of TERT gene transfected BMSC on memory function and hippocampal CA1 region synaptic plasticity in vascular dementia rat
摘要
Abstract
Objective To explore the effect of telomerase reverse transcriptase(TERT) gene transfected bone marrow stem cell(BMSC)on the memory function and hippocampal CA1 region synaptic plasticity in vascular dementia rat.Methods A total of 60 rats were randomly divided into the negative control group(group A),model group(group B),conventional BMSC group(group C) and transfected BMSC group(group D).The related indicators in each group were detected by using the Morris maze test,RTPCR and Western blot respectively.Results The escape latency period in the group C and group D was significantly longer than that in the group B,which in the group D was significantly longer than that in the group C.Compared with the group A,the expressions of brain-derived neurotrophic factor(BDNF)mRNA,TERT mRNA,SYP mRNA and protein in the group B,group C and group D were significantly decreased.The synaptic cleft arrange in group A was clear with more SYN positive ceils.The synaptic cleft in the group D was clearer,and the number of SYN positive cells was close to that in group A.Conclusion TERT transfected BMSC has obvious therapeutic effect on vascular dementia rats and its mechanism may be related to the promotion of BDNF,TrkB expression and the improvement of synaptic plasticity.关键词
端粒酶逆转录酶/骨髓基质干细胞/痴呆,血管性/记忆功能/突触/可塑性Key words
telomerase reverse transcriptase/bone marrow stromal stem cells/dementia, vascular/memory function/synaptic/plasticity分类
医药卫生引用本文复制引用
段金旗,马丽琼,刘远林,任炜,陈媛媛,李春岩..TERT基因转染BMSC血管性痴呆大鼠记忆功能及海马CA1区突触可塑性的影响[J].重庆医学,2017,46(10):1300-1303,1307,5.基金项目
国家自然科学基金资助项目(81171210). （81171210）
