动物营养学报2016，Vol.28Issue(6)：1677-1686,10.DOI:10.3969/j.issn.1006-267x.2016.06.007
笼养和散养蛋鸡小肠细菌菌群区系的聚合酶链式反应-变性梯度凝胶电泳分析
Intestinal Bacterial Flora of Chicken Fed in Cage and Free Range by Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis Analysis
摘要
Abstract
This study investigated the structural diversity of intestinal bacterial flora in young and adult chicken fed in cage and free range separately.Intestinal samples were collected from 8 and 30 weeks old chickens.Polymerase chain reaction and denaturing gradient gel electrophoresis (PCR-DGGE) was used,in combination with cloning and sequencing of amplified fragments to produce bacteriai flora DNA fingerprints.Total of 39 strains were isolated from the intestinal contents of chicken,including 4 sulfer bacteria as 2 Proteobacteria strains (5.1％),4 Bacteroidetes strains (10.2％),5 Actinobacteria strains (12.8％) and 21 Firmicutes strains (53.8％),as well as 7 environmental samples (uncultured bacterium).And bacteria species were various in different intestine parts of different chicken,bacteria in adult hens and chicken fed in free range was richer than bacteria in young chicken and chicken fed in cage.Ten Lactobacillus stains were isolated.Coprococcus was excluded but the rest of nine Lactobacillus species were saved to study for probiotics.Results indicate that feeding mode and chicken age have a great influence on the intestinal bacterial community composition,and more bacteriai species exist in adult hens' intestinal tract and fed in free range.关键词
蛋鸡/笼养/散养/PCR-DGGE/菌群鉴定Key words
chicken/cage range/free range/PCR-DGGE/flora identification分类
农业科技引用本文复制引用
崔一喆,王秋菊,李悦,苏景,周亚强,张宇辰..笼养和散养蛋鸡小肠细菌菌群区系的聚合酶链式反应-变性梯度凝胶电泳分析[J].动物营养学报,2016,28(6):1677-1686,10.基金项目
黑龙江省自然科学基金项目(C201444,C2015040) （C201444,C2015040）
黑龙江八一农垦大学校培育课题(XZR2014-05) （XZR2014-05）
