动物营养学报2016，Vol.28Issue(6)：1720-1725,6.DOI:10.3969/j.issn.1006-267x.2016.06.012
热激条件下家蚕血液30K蛋白磷酸化研究
Study on 30K Protein Phosphorylation of Silkworm Haemolymph under Heat Shock
摘要
Abstract
This experiment was conducted to investigate the expression changes of phosphorylated protein in silkworm haemolymph under heat shock,and provided a reference for further study of its function.Two groups were designed,and 30 fifth-instar silkworms (half male and half female) were randomly divided in each group,and treated with heat shock under 40 ℃ for 0 (control group) and 10 min (heat shock group),respectively.The differentially expressed phosphorylation proteins were studied utilizing two-dimensional electrophoresis,Pro-Q Diamond dye and mass spectrometry.The results showed as follows:1) amount of proteins were accumulated in the 30 ku area of silver staining map and revealed as phosphorylated proteins by Pro-Q Diamond dyeing.2) Three 30K proteins were identified as PBMHPC-6,PBMHPC-19 and PBMHPC-12 by multiple sampling and mass spectrometry identification.3) Compared with 0 min heat shock,the expression levels of three 30K proteins were significantly up-regulated after 10 min heat shock (P＜0.05).It is concluded that fifthinstar silkworm haemolymph are rich in 30K proteins.These proteins not only play important roles in lipid transport,but also may involve in heat shock response.关键词
热激/家蚕/血液/30K蛋白/磷酸化/双向电泳Key words
heat shock/silkworm/haemolymph/30K proteins/phosphorylation/two-dimensional electrophoresis分类
农业科技引用本文复制引用
李娜,贾漫丽,杨贵明,李季生..热激条件下家蚕血液30K蛋白磷酸化研究[J].动物营养学报,2016,28(6):1720-1725,6.基金项目
河北省教育厅资助项目(Q2012026,ZD2016044) （Q2012026,ZD2016044）
