动物营养学报2016，Vol.28Issue(6)：1726-1734,9.DOI:10.3969/j.issn.1006-267x.2016.06.013
饲粮阴阳离子差水平对小鼠血钙浓度及胃肠道组织钙结合蛋白-D9k mRNA相对表达水平的影响
Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Tissues Calcium Binding Proteins-D9k mRNA Relative Expression Level of Mice during the Transition Period
摘要
Abstract
Based on the calcium related gene expression profile and its analysis,the study was conducted to investigate the effects of dietary cation-anion difference (DCAD) level on plasma calcium concentration and gastrointestinal tract tissues calcium binding proteins-D9k (CaBP-D9k) relative expression level of mice during the transition period,and to clarify the mechanism of low DCAD level prevent hypocalcaemia.One hundred and twenty KM mice were randomly allotted into 3 groups with 40 mice in each group,mice in the 3 groups were fed 3 diets with DCAD level at +300 (high DCAD level group,HD group),+150 (control group,CON group),and-150 (low DCAD level group,LD group),respectively.Plasma calcium concentration and gastrointestinal tract tissues CaBP-D9k mRNA relative expression level were detected at 4 time points:day of 20 (-20 d) and 5 (-5 d) before kidding,day of kidding (0 d),and day of 3 after kidding (+3 d).The results showed that compared with the HD group,the plasma calcium concentration of mice at 0 d and +3 d during the transition period in LD group was significantly increased (P＜0.05),and the CaBP-D9k mRNA relative expression level was significantly up-regulated in duodenum,jejunum,ileum and colon (P＜0.05),especially observed on-5 d before parturition and 0 d at parturition (P＜0.05).Also,CaBP-D9k mRNA relative expression level was significantly increased in jejunum and colon on the +3 d after parturition (P＜0.05).Moreover,there was a significant correlation between CaBP-D9k mRNA relative expression level in duodenum,jejunum and colon and plasma calcium concentration.In conclusion,low DCAD level is able to up-regulate CaBP-D9k mRNA relative expression level in small intestine and colon,accompany with greater plasma calcium concentration.This is possibly an important way of low DCAD level maintains plasma calcium homeostasis during the transition period,and reduce the occurrence rate of hypocalcemia.关键词
饲粮阴阳离子差/血钙浓度/钙结合蛋白/胃肠道组织Key words
dietary cation-anion difference/plasma calcium concentration/calcium-binding protein/gastrointestinal tract tissues分类
农业科技引用本文复制引用
吴文旋,吴佳海,张吉鹍,莘海亮,杨艺,朱伦琴..饲粮阴阳离子差水平对小鼠血钙浓度及胃肠道组织钙结合蛋白-D9k mRNA相对表达水平的影响[J].动物营养学报,2016,28(6):1726-1734,9.基金项目
国家自然科学基金(31360563) （31360563）
贵州大学科技兴村人才培育基地建设项目 （）
贵州山区牧草产业化生产技术研究集成与应用(黔科合重大专项字[2014]6017号) （黔科合重大专项字[2014]6017号）
动物营养学国家重点实验室开放课题(2004DA125184F1115) （2004DA125184F1115）
贵州省农业科技攻关项目(黔科合NY字[2009]3085) （黔科合NY字[2009]3085）
