动物营养学报2016，Vol.28Issue(6)：1887-1898,12.DOI:10.3969/j.issn.1006-267x.2016.06.032
黄芪多糖对种公鸡不同组织miR-16表达的影响及其功能预测分析
Effects and Bioinformatic Analysis of Astragalus Polysaccharide on miR-16 Expression in Different Tissue of Breeder Cocks
摘要
Abstract
This experiment was conducted to investigate the effects of Astragalus polysaccharides (APS) on miR-16 expression in different tissues of breeder cocks,combining with the functional enrichment analysis of GO and KEGG pathway of miR-16 predicted targets,and to explore the potential regulation role of APS in different tissues via miR-16.A total of 64 one-day-old Cobb 500 breeder cocks were randomly divided into control group and APS group with 4 replicates per group and 8 breeder cocks per replicate.Cocks in the control group were fed a corn-soybean meal based diet,and the others in APS group were fed the basal diet supplemented with 10 g/kg APS.The experiment lasted for 40 weeks.The results showed as follows:1) compared with the control group,dietary supplemented with APS improved the semen volume and number of effective sperm,decreased the percentage of abnormal sperm (P＞0.05).2) miR-16 differentially expressed in different tissues;APS ssignificantly increased the miR-16 expression in liver,spleen,duodenum mucosa and ileum mucosa (P＜0.05 or P＜0.01),and down-regulated miR-16 expression in testis (P＜0.05).3) GO enrichment analysis of miR-16 predicted that targets were showed to be enriched for the metabolic functions in transmembrane transport,ubiquitin-dependent protein catabolic process,intracellular protein transport,glycolytic process (P＜0.05 or P＜0.01).KEGG pathway showed that the target genes were enriched in focal adhesion,insulin signaling pathway,glycolysis/gluconeogenesis associated with cell proliferation,differentiation and metabolism (P＜0.05).And the target genes were also enriched in toll-like receptors signaling pathways and natural killer cell mediated cytotoxicity related to immune pathways.In conclusion,APS up-regulates the expression of miR-16 in liver,spleen,duodenum mucosa,ileum mucosa,while down-regulates that in testis of breeder cocks.miR-16 predicted targets collect with metabolism and immune regulation,it showes that APS regulated the miR-16 expression on different tissues of breeder cocks to affect the metabolism and immune regulation functions.关键词
黄芪多糖/种公鸡/miR-16/精液品质/组织表达/功能分析Key words
APS/breeder cocks/miR-16/semen quality/tissue expression/bioinformatics analysis分类
农业科技引用本文复制引用
任小春,李玉龙,武圣儒,雷新宇,郭伟,党燕娜,龚月生,杨小军..黄芪多糖对种公鸡不同组织miR-16表达的影响及其功能预测分析[J].动物营养学报,2016,28(6):1887-1898,12.基金项目
国家自然科学基金项目(31272464) （31272464）
教育部新世纪优秀人才项目(NCET-12-0476) （NCET-12-0476）
陕西省农业公关计划(2014K01-18-02,2015NY149) （2014K01-18-02,2015NY149）
陕西省科技统筹创新工程计划项目(2015KTCQ02-19) （2015KTCQ02-19）
