动物营养学报2016，Vol.28Issue(6)：1899-1906,8.DOI:10.3969/j.issn.1006-267x.2016.06.033
颈静脉灌注精氨酸对泌乳中期奶牛血清生化和免疫指标的影响
Effects of Arginine Infusion through Jugular Vein on Serum Biochemical and Immune Indices of Dairy Cows at Mid-Lactation
摘要
Abstract
This study was conducted to investigate the effects of arginine infusion through jugular vein on serum biochemical and immune indices of dairy cows at mid-lactation.A 3 × 3 Latin square trail was used.Six healthy lactating cows at similar lactation stages with similar body weight,parities,days in milk,milk yield and body condition were selected and divided into 3 groups (2 for each group),which were casein group (control group),arginine group (infused 37.66 g/d),and alanine group (equal nitrogen to arginine group,infused 77.24 g/d);three periods of test were carried out,and each period lasted for 22 d (7-day infusion period plus 15-day interval period).Blood was collected before morning feeding at the last day of infusion period to detect serum biochemical and immune indices.The results showed as follows:the concentrations of total protein,immunoglobulin G (IgG),tumor necrosis factor α (TNF-α) in serum in arginine group were significantly higher than those in other groups (P＜0.05),the concentrations of interleukin 1 (IL-1) and the ratio of albumin to globulin in arginine group were significantly higher than those in alanine group (P＜0.05),and the number of somatic cells in milk in arginine group was significantly lower than that in other 2 groups (P ＜ 0.05),but no significant differences were found in the activities of gluamic-pyruvic transaminase and glutamic oxalacetic transaminase,and the concentrations of immunoglobulin A (IgA),immunoglobulin E (IgE) and intedeukin 6 (IL-6) among groups (P＞0.05).In conclusion,arginine infusion can promote immunity performance of lactating cows and the passive immunity of fetus in some degree by increasing serum total protein,IgG,IL-1 and TNF-α concentrations.关键词
颈静脉灌注/精氨酸/免疫球蛋白/细胞因子/体细胞数Key words
jugular vein infusion/arginine/immune globulin/cytokine/somatic cells分类
农业科技引用本文复制引用
丁洛阳,梁祥焕,王梦芝,张军,王洪荣,周刚,张鑫,徐巧云..颈静脉灌注精氨酸对泌乳中期奶牛血清生化和免疫指标的影响[J].动物营养学报,2016,28(6):1899-1906,8.基金项目
江苏省自然科学基金基础研究项目(BK20141270,BK20151312) （BK20141270,BK20151312）
江苏省高校研究生创新工程项目(KYLX14_1358) （KYLX14_1358）
江苏省优势学科(PAPD) （PAPD）
