动物营养学报2016，Vol.28Issue(6)：1956-1964,9.DOI:10.3969/j.issn.1006-267x.2016.06.039
含钒蛋黄粉对大鼠生长、氧化应激状态及其相关基因表达的影响
Effects of Egg Yolk Powder Including Vanadium on Growth, Oxidative Stress Status and Its Related Gene Expression of Wistar Rats
摘要
Abstract
As a kind of heavy metal,excessive intake of vanadium can cause oxidative stress to laying hens,reduce egg quality and make residue in eggs,which affects the safety of eggs.The objective of this study was to explore the effects of feeding egg yolk powder including vanadium to Wistar rats on their growth,oxidative stress status and its related gene expression,and to evaluate the biosecurity of organic vanadium.A total of 27 female Wistar rats (4 weeks old) were allocated to 3 treatments with 9 rats each,and fed three kinds of diets supplemented with 600 g/kg egg yolk powders which were prepared with eggs from laying hens fed diets containing 0,5 and 10 mg/kg vanadium,respectively.The three diets actually contained 0.107,0.137 and 0.164 mg/kg vanadium.The experiment lasted for 35 d.The results showed that there were no significant differences on growth performance,organ indices,contents of plasma triglyceride,malonaldehyde (MDA) and urea nitrogen,activities of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST),liver and kidney superoxide dismutase (SOD) activity,MDA content,and total antioxidant capacity (T-AOC),activities of liver ALT and AST,liver glutathione content,and liver glutathione S-transferase activity,as well as histomorphology of liver and kidney and vanadium residual in kidney of rats among 3 treatments (P＞0.05).However,compared with treatment 1,liver quinone oxidoreductase 1 (NQO1) activity of rats in treatments 2 and 3 was significantly decreased (P＜0.05),and the relative expression levels of liver NQO1 and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA were significantly decreased (P＜0.05),too.The results indicate that the dietary supplementation of 600 mg/kg egg yolk powders with different vanadium content has no significantly different effects on growth performance and oxidation-reduction state of Wistar rats.However,the NQO1 activity,mRNA expression of NQO1 and Nrf2 are down-regulated as the vanadium content increasing (0.137 and 0.164 mg/kg).关键词
蛋黄粉/钒/大鼠/氧化应激/醌氧化还原酶1/核因子E2相关因子2Key words
egg yolk powder/vanadium/rat/oxidative stress/NQO1/Nrf2分类
农业科技引用本文复制引用
崔仁勇,王建萍,张克英,丁雪梅,曾秋凤,白世平,罗玉衡..含钒蛋黄粉对大鼠生长、氧化应激状态及其相关基因表达的影响[J].动物营养学报,2016,28(6):1956-1964,9.基金项目
四川省科技支撑项目(2011NZ0073,2014NZ0002):科技部科技支撑项目(2014BAD13B04) （2011NZ0073,2014NZ0002）
四川省教育厅项目(13ZB0290) （13ZB0290）
