南方医科大学学报2017，Vol.37Issue(4)：423-430,8.DOI:10.3969/j.issn.1673-4254.2017.04.01
间歇性断食对老年前期肥胖大鼠肠道菌群及代谢的影响
Effect of intermittent fasting on physiology and gut microbiota in presenium rats
摘要
Abstract
Objective To investigate the effect of intermittent fasting on metabolize and gut microbiota in obese presenium rats fed with high-fat-sugar-diet. Methods We fed the Wistar rats with high-fat and high-sugar diet to induce adiposity, and the rats for intermittent fasting were selected base on their body weight. The rats were subjected to fasting for 72 h every 2 weeks for 18 weeks. OGTT test was performed and fasting blood samples and fecal samples were collected for measurement of TC, TG, HDL-C and LDL-C and sequence analysis of fecal 16S rRNA V4 tags using Illumina. Gut microbial community structure was analyzed with QIIME and LEfSe. Results After the intervention, the body weight of the fasting rats was significantly lower than that in high-fat diet group (P<0.01). OGTT results suggested impairment of sugar tolerance in the fasting group, which showed a significantly larger AUC than compared with the high-fat diet group (P<0.05). Intermittent fasting significantly reduced blood HDL-C and LDL-C levels (P<0.05) and partially restored liver steatosis, and improved the gut microbiota by increasing the abundance of YS2, RF32 and Helicobacteraceae and reducing Lactobacillus, Roseburia, Erysipelotrichaceae and Ralstonia. Bradyrhizobiaceae was found to be positively correlated with CHOL and HDL-C, and RF39 was inversely correlated with the weight of the rats. Conclusion Intermittent fasting can decrease the body weight and blood lipid levels and restore normal gut microbiota but can cause impairment of glucose metabolism in obese presenium rats.关键词
肥胖/断食/肠道菌群/16SrRNAKey words
obesity/fasting/gut microbiota/16S rRNA引用本文复制引用
容祖华,周宏伟,梁少聪,路浚齐,何彦,罗月梅,游超,夏耿红,Prabhakar M,李攀..间歇性断食对老年前期肥胖大鼠肠道菌群及代谢的影响[J].南方医科大学学报,2017,37(4):423-430,8.基金项目
国家自然科学基金优秀青年基金(31322003) Supported by National Natural Science Foundation of China (31322003). （31322003）
