南方医科大学学报2017，Vol.37Issue(4)：431-437,7.DOI:10.3969/j.issn.1673-4254.2017.04.02
河蚌葡聚糖对半月板组织损伤及病理性过度肥大和钙化的影响
Effect of HBP-A on meniscal injury and pathological hypertrophy and calcification of the meniscus
摘要
Abstract
Objective To investigate the effect of HBP-A on meniscal injuries and the expressions of genes associated with pathological hypertrophy and calcification of the meniscusinduced by abnormal loading. Methods Bovine meniscus explants were subjected to 25%strain at 0.3 Hz for 3 h and treated with 0.6 mg/mL of HBP-A. The cell viability in the meniscus explants after 72 hin culture was determined using live/dead staining and the expression levels of genes associated with pathological hypertrophy and calcification of the meniscus (ANKH, ENPP1, ALP, MMP13, and IL-1) were measured using real-time PCR and Western blotting. The conditioned medium was collected for testing sulfated glycosaminoglycan (GAG) release. Results The number of dead cells, loss of proteoglycan content, and the expressions of ANKH, ENPP1, ALP and MMP13, and IL-1 at both the mRNA and protein levels were all significantly lower in the meniscus explants treated with 0.6 mg/mL HBP-A than in the explants with only 25%abnormal pressure stimulation (n=3, P<0.05). Conclusion HBP-A can effectively alleviate meniscal injuries induced by abnormal loading and suppress the expressions of genes related with pathological hypertrophy and calcification of the meniscus, and can serve as a potential drug for treatment of knee osteoarthritis.关键词
河蚌葡聚糖/异常压力/刺激/半月板肥大/钙化Key words
HBP-A/abnormal loading/meniscus/hypertrophy/calcification引用本文复制引用
杜国庆,丁道芳,冯媛媛,李玲慧,雷腾飞,陈博,邓真,詹红生..河蚌葡聚糖对半月板组织损伤及病理性过度肥大和钙化的影响[J].南方医科大学学报,2017,37(4):431-437,7.基金项目
国家自然科学基金(81473702,81674005) （81473702,81674005）
国家自然科学基金青年科学基金(81503596,81202707,81001528) （81503596,81202707,81001528）
北京市自然科学基金青年项目(7164313) （7164313）
中医骨伤科学"国家重点学科(100508) （100508）
上海市中医药领军人才建设项目(2012-63-15) （2012-63-15）
海派中医流派传承研究基地(ZY3-CCCX-1-1003) （ZY3-CCCX-1-1003）
上海市教育委员会高峰高原建设中医骨伤团队 （）
张江高科技园区中医药发展扶持项目(pzy2015-1) Supported by National Natural Science Foundation of China (81473702, 81674005). （pzy2015-1）
