南方医科大学学报2017，Vol.37Issue(4)：438-443,6.DOI:10.3969/j.issn.1673-4254.2017.04.03
脓毒症大鼠通过降低肌浆网钙摄取和SERCA1表达损害膈肌舒张功能
Lowered sarcoendoplasmic reticulum calcium uptake and diaphragmatic SERCA1 expression contribute to diaphragmatic contractile and relaxation dysfunction in septic rats
摘要
Abstract
Objective The explore the mechanism responsible for diaphragmatic contractile and relaxation dysfunction in a rat model of sepsis. Methods Thirty-six adult male Sprague-Dawley rats were randomized equally into a sham-operated group and two model groups of sepsis induced by cecal ligation and puncture (CLP) for examination at 6 and 12 h following CLP (CLP-6 h and CLP-12 h groups). The parameters of diaphragm contractile and relaxation were measured, and the calcium uptake and release rates of the diaphragmatic sarcoendoplasmic reticulum (SR) and the protein expressions of SERCA1, SERCA2 and RyR in the diaphragmatic muscles were determined. Results The half-relaxation time of the diaphragm was extended in both the CLP-6 h and CLP-12 h groups with significantly reduced maximum tension declinerate and the peek uptake rate of SERCA (P<0.01). Diaphragmatic maximum twitch force development rate, the maximal twitch, tetanus tensions and the peek release rate of SR decreased only at 12h after CLP (P<0.01). The expression levels of SERCA1 protein decreased significantly in the diaphragmatic muscles at 12h following CLP (P<0.01) while SERCA2 expression level and SERCA activity showed no significant changes. Conclusion In the acute stage of sepsis, both the contractile and relaxation functions of the diaphragm are impaired. Diaphragmatic relaxation dysfunction may result from reduced calcium uptake in the SR and a decreased level of SERCA1 in the diaphragmatic muscles.关键词
脓毒症/急性期/膈肌/肌浆网钙摄取率/Ca2+-ATP酶Key words
sepsis/calium uptake/diaphragm/SERCA/sarcoendoplasmic reticulum/muscle relaxation引用本文复制引用
张建友,吴进,李士通,龚园..脓毒症大鼠通过降低肌浆网钙摄取和SERCA1表达损害膈肌舒张功能[J].南方医科大学学报,2017,37(4):438-443,6.基金项目
国家自然科学基金(81171845) （81171845）
上海自然科学基金(14ZR1438100) Supported by National Natural Science Foundation of China (81171845). （14ZR1438100）
