南方医科大学学报2017，Vol.37Issue(4)：475-481,7.DOI:10.3969/j.issn.1673-4254.2017.04.09
抑制热休克蛋白70表达可增强宫颈癌细胞对顺铂化疗的敏感性
Inhibiting HSP70 expression enhances cisplatin sensitivity of cervical cancer cells
摘要
Abstract
Objective To investigate the relationship between sensitivity to cisplatin (DDP) and the expression of HSP70 in cervical cancer cells in vitro. Methods Cervical cancer Hela229 cells treated with different concentrations of DDP and the HSP70 inhibitor (PFT-μ) were examined for cell viability using MTT assay and colony forming ability. The cell apoptosis was analyzed by flow cytometry with propidium iodide staining and DAPI staining, and JC-1 staining was used to determine mitochondrial membrane potential. The expressions of HSP70, Bcl-2, Bax and caspase-3 were measured with Western blotting. A nude mouse model bearing Hela229 cell xenograft was used to evaluate the effect of DDP and PFT-μ on tumor growth. Results Hela229 cells expressed a higher level of HSP70 than normal cervical cells. The combined use of PFT-μ significantly enhanced the inhibitory effect of DDP (P<0.01) and increased the cell apoptosis in Hela229 cells. JC-1 staining demonstrated that DDP combined with PFT-μmore obviously reduced mitochondrial membrane potential. DDP combined with PFT-μmore strongly lowered Bcl-2 expression and increased the expressions of casepase-3 and Bax than DDP alone. In the nude mouse model, PFT-μ significantly enhanced DDP sensitivity of Hela229 cell xenografts (P<0.01). Conclusions Inhibition of HSP70 expression can enhance the sensitivity of cervical cancer cell to DDP both in vivo and in vitro possibly by promoting cell apoptosis, suggesting the potential of HSP70 as a new target for gene therapy of cervical cancer.关键词
热休克蛋白70/宫颈癌/热休克蛋白70抑制剂/顺铂Key words
heat shock protein 70/cervical cancer/PFT-μ/cisplatin引用本文复制引用
刘健,刘静,李胜泽,郑迎奥,郭苏阳,王秀..抑制热休克蛋白70表达可增强宫颈癌细胞对顺铂化疗的敏感性[J].南方医科大学学报,2017,37(4):475-481,7.基金项目
安徽高校省级自然科学研究一般项目(KJ2015B096by) （KJ2015B096by）
