南方医科大学学报2017，Vol.37Issue(4)：555-562,8.DOI:10.3969/j.issn.1673-4254.2017.04.24
27重SNP系统推断种族来源的效能
Efficiency of 27-plex single nucleotide polymorphism multiplex system for ancestry inference in different populations
摘要
Abstract
Objective To validate the efficiency of 27-plex single nucleotide polymorphism (SNP) multiplex system for ancestry inference. Methods The 27-plex SNP system was validated for its sensitivity and species specificity. A total of 533 samples were collected from African, Southern Chinese Han, China's ethic minorities (Yi, Hui, Miao, Tibet, and Uygur), European, Central Asian, Western Asian, Southern Asian, Southeast Asian and South American populations for clustering analysis of the genotypes by citing 3 representative continental ancestral groups [East Asia (CHB), Europe (CEU), and Africa (YRI)] from HapMap database. Results The system sensitivity is 0.125 ng. Twenty and six genotypes were detected in chimpanzee and monkeys, respectively. Except in rs10496971, no more products were found in other animals. The system was capable of differentiating intercontinental populations but not of distinguishing between East Asian and Southeast Asian population or between Southern Chinese Han population and Chinese Ethnic populations (Hui, Miao, Yi and Tibet). This system achieved a 100%accuracy for intercontinental population source inference for 46 blind test samples. Conclusion 27-plex SNPs multiplex system has a high sensitivity and species specificity and can correctly differentiate the ancestry origins of individuals from African, European and East Asian for criminal case investigation. But this system is not capable of distinguishing subpopulation groups and more specific ancestry-informative markers are needed to improve its recognition of Southeast Asian and Chinese ethnic populations.关键词
法医物证学/SNP/祖先信息位点/种族来源推断Key words
forensics biological evidence/single nucleotide polymorphisms/ancestry-informative markers/ancestry inference引用本文复制引用
冯杏玲,孙启凡,刘宏,魏以梁,杜蔚安,李彩霞,陈玲,刘超..27重SNP系统推断种族来源的效能[J].南方医科大学学报,2017,37(4):555-562,8.基金项目
法医遗传学公安部重点实验室开放课题(2015FGKFKT03) （2015FGKFKT03）
"十二五"国家科技支撑计划项目(2012BAK02B01-1) （2012BAK02B01-1）
