福建农林大学学报（自然科学版）2017，Vol.46Issue(2)：166-171,6.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2017.02.008
水稻苗期耐盐性基因SST分子标记的筛选与应用
Screening and application of molecular marker for salt tolerance gene SST at rice seedling stage
摘要
Abstract
Molecular markers, INDEL1 and INDEL2, which are linked to salt tolerance gene SST of rice seedling, were designed. The specificity and reliability of 5 markers, including ID27093, ID27101, ID27118, INDEL1, and INDEL2, were analyzed through screening 67 rice varieties and a F2 population from a cross between mutant sst and a rice restorer line Huazhan for salt tolerance, and mutant sst was used as a salt tolerance control. The results showed that all markers except INDEL2 demonstrated low polymor-phism. INDEL2 turned out to be a co-dominant marker with high specificity, and it was able to distinguish from 85.07% of the tested parents and mutant sst with high screening accuracy. Therefore, marker INDEL2 could be directly used as a molecular marker in breeding for rice seedling salt tolerance gene SST.关键词
水稻/耐盐性基因SST/分子标记/筛选Key words
Oryza sativa L./salt tolerance gene SST/molecular maker/screening分类
农业科技引用本文复制引用
彭江涛,候新坡,兰涛,毛大梅,陈志伟,潘润森,官华忠..水稻苗期耐盐性基因SST分子标记的筛选与应用[J].福建农林大学学报（自然科学版）,2017,46(2):166-171,6.基金项目
福建省科技重大专项(2013NZ0002-2) （2013NZ0002-2）
福建省自然科学基金(2012J01091) （2012J01091）
福建省教育厅科技计划项目(JA12095). （JA12095）
