分析测试学报2017，Vol.36Issue(2)：190-195,6.DOI:10.3969/j.issn.1004-4957.2017.02.006
脑啡肽的固相合成与LC-MS/MS分离鉴定
Separation and Characterization of Solid Phase Synthesized Enkephalins by LC-MS/MS
摘要
Abstract
Peptides play an important role in life process.In this paper,two endogenous neuropeptides including methionine enkephalin and leucine enkephalin were selected as research targets.They were synthesized by Fmoc solid phase peptide synthesis strategy.An HPLC-ESI-MS/MS method was established to investigate the composition of synthetic peptides.The possible structure modifications during peptide synthesis procedures were also identified.The results showed that the main byproducts were incomplete peptides and oxidative peptides.Solutions for high efficient synthesis of methionine enkephalin and leucine encephalin were further proposed.This study provides new perspectives in rapid peptide structure identification and efficient bioactive peptide synthesis.关键词
多肽固相合成/甲硫氨酸脑啡肽/亮氨酸脑啡肽/氧化/液相色谱-质谱联用Key words
solid phase peptide synthesis/methionine enkephalin/leucine enkephalin/oxidation/high performance liquid chromatography tandem mass spectrometry(LC-MS/MS)分类
化学化工引用本文复制引用
瑙甘,金钰龙,黄嫣嫣,赵睿..脑啡肽的固相合成与LC-MS/MS分离鉴定[J].分析测试学报,2017,36(2):190-195,6.基金项目
国家自然科学基金资助项目(21375134,21475140) （21375134,21475140）
