分析测试学报2017，Vol.36Issue(2)：276-279,4.DOI:10.3969/j.issn.1004-4957.2017.02.022
鱼组织中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素含量的超高效液相色谱法测定
Simultaneous Determination of Bisdemethoxycurcumin, Demethoxycurcumin and Curcumin in Fish Tissues by UPLC
摘要
Abstract
An original method for the simultaneous determination of bisdemethoxycurcumin,demethoxycurcumin and curcumin in plasma,muscle,liver and kidney of yellow catfish(Pelteobagrus fulvidraco),bluntnose black bream (Megalobrama amblycephala) and grass carp (Ctenopharyngodon idellus) was established by ultra high performance liquid chromatography with ultraviolet detection (UPLC-UV).The sample was extracted using acetonitrile containing 0.01％ acetic acid,and the moisture of sample was removed with anhydrous sodium sulfate,then n-hexane was used for removing fat in sample.After that,the sample prepared was separated on an ACQUIT UPLC BEH C18 column and determined at 428 nm.Good linearities were observed in the range of 0.01-5.00 mg/L,and the correlation coefficients for bisdemethoxycurcumin,demethoxycurcumin and curcumin were 0.998 7,0.999 8 and O.999 6,respectively.The average recoveries of 3 analytes in fish tissues at the spiked level of 0.025-1.00 mg/kg ranged from 64.7％ to 102.2％,with relative standard deviations(RSDs) of 0.69％-10.8％.The detection limits(LODs) and quantitation limits(LOQs) for 3 analytes in fish tissues were all 0.010 mg/kg and 0.025 mg/kg,respectively.The presented method was firstly applied in the investigation on the pharmacokinetic profile of curcumin in bluntnose black bream.关键词
鱼组织/双去甲氧基姜黄素/去甲氧基姜黄素/姜黄素/超高效液相色谱Key words
fish tissues/bisdemethoxycurcumin/demethoxycurcumin/curcumin/ultra high performance liquid chromatography (UPLC)分类
化学化工引用本文复制引用
刘永涛,李乐,王赛赛,余琳雪,杨秋红,杨移斌,艾晓辉..鱼组织中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素含量的超高效液相色谱法测定[J].分析测试学报,2017,36(2):276-279,4.基金项目
现代农业技术体系专项资金(CARS-49) （CARS-49）
中国水产科学研究院基本科研业务费资助(2016JBF0104) （2016JBF0104）
公益性行业(农业)科研专项资助(201503108-CC-1) （农业）
