分析化学2017，Vol.45Issue(2)：211-216,6.DOI:10.11895/j.issn.0253-3820.160460
靶向亲和-液相色谱-质谱联用技术快速筛选黄藤总生物碱中乙酰胆碱酯酶抑制剂
Screening and Structure Characterization of Acetylcholinesterase Inhibitors from Total Alkaloids of Fibraurea recisa Pierre.by Target Molecule Affinity-Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A target molecule affinity-ultrafiltration liquid chromatography-electrospray ionization tandem mass spectrometric (LC-ESI-MSn) method was established for rapid screening acetylcholinesterase (AChE) inhibitors from total alkaloids of fibraurea recisa Pierre.A total of 12 potential inhibitors were screened from Fibraurea recisa Pierre.and 6 compounds were identified including palmatine,berberine,jatrorrhizine,palmatrubine,7,8-dihydro-8-hydroxyberberine and groenlandicine.The AChE inhibitory activity of these 6 compounds was validated in vitro.Palmatine showed the strongest inhibitory activity for AChE,which was stronger than that of donepezil hydrochloride,demonstrating the potential of palmatine as anti-Alzheimer's drug.This method is simple,rapid,and accurate for directly screening active ingredients which can inhibit AChE from complex extract of traditional Chinese medicines.关键词
靶向亲和-液相色谱-质谱联用技术/黄藤总生物碱/乙酰胆碱酯酶/抑制剂Key words
Target molecule affinity-liquid chromatography-tandem mass spectrometry/Total alkaloids of Fibraurea recisa Pierre./Acetylcholinesterase/Inhibitor引用本文复制引用
何忠梅,吕娜,南敏伦,赵昱玮,赫玉芳,孟令文,孙佳明,张连学..靶向亲和-液相色谱-质谱联用技术快速筛选黄藤总生物碱中乙酰胆碱酯酶抑制剂[J].分析化学,2017,45(2):211-216,6.基金项目
本文系吉林省中医药产业发展专项(No.20140311029YY),吉林省教育厅双十项目(No.201442)资助项目 （No.20140311029YY）
