口腔疾病防治2017，Vol.25Issue(4)：223-226,4.DOI:10.12016/j.issn.2096-1456.2017.04.004
槟榔碱对人牙周膜成纤维细胞凋亡的影响
Effects of arecoline on apoptosis of human periodontal ligament fibroblasts
王焕玲 1张建平2
作者信息
- 1. 天津市河东区鲁山道社区卫生服务中心,天津 300171
- 2. 天津中医药大学第二附属医院,天津 300171
- 折叠
摘要
Abstract
Objective To observe the effects of arecoline on the related protein expressions in the apoptosis of cul-tured human periodontal ligament fibroblasts (hPDLFs), such as p-JNK, p-p53, and Bcl-2. Methods hPDLFs were iso-lated from human periodontal ligament tissues and expanded in vitro. hPDLFs were treated with different concentrations of arecoline (0, 20, 40 and 80 μg/mL) for 12 h. The proliferative activities were evaluated by MTT. The expressions of p-JNK, p-p53, and Bcl-2 were determined by Western blot. Results Arecoline inhibited cell proliferation and induced apoptosis protein. The protein level of Bcl-2 was decreased, while p-JNK and p-p53 were increased (P<0.05). The pro-tein expressions were in a concentration-dependent manner with arecoline. Conclusion It demonstrates arecoline might contribute to the apoptosis of hPDLFs, and could destroy periodontal tissues.关键词
槟榔碱/人牙周膜成纤维细胞/凋亡/抑制/蛋白表达Key words
Arecoline/Human periodontal ligament fibroblasts/Apoptosis/Inhibition/Protein expression分类
医药卫生引用本文复制引用
王焕玲,张建平..槟榔碱对人牙周膜成纤维细胞凋亡的影响[J].口腔疾病防治,2017,25(4):223-226,4.
