影像科学与光化学2016，Vol.34Issue(5)：435-443,9.DOI:10.7517/j.issn.1674-0475.2016.05.435
比率型SO2荧光探针的构建及细胞成像研究
Construction of a Ratiometric Fluorescent Probe for SO2 Derivative and Its Application for Cell Imaging
摘要
Abstract
In this study,we report a ratiometric fluorescent probe (CZBI) for sulfur dioxide (SO2) derivatives based on the conjugate of carbazole and benzo[e]indolium,which displays colorimetric and ratiometric fluorescence dual response to HSO3-/SO32-.The probe can quantitatively detect HSO3 with high specificity,fast response (within 40 s) as well as low detection limit (12 nmol/L).The fluorescence intensity ratios were linearly related to the concentrations of bisulfite ranging from 0 to 16 μmol/L.A 1,4-nucleophilic addition reaction was proposed for the sensing mechanism of this probe,which was confirmed by 1HNMR spectra.Fluorescence imaging of HeLa cells indicated that CZBI could be used for monitoring the intrinsically generated intracellular SO2 derivatives in living cells by ratiometric fluorescence imaging.关键词
荧光探针/SO2/苯并吲哚/比率/细胞成像Key words
fluorescent probe/SO2/benzo[e]indole/ratiometric/cell imaging引用本文复制引用
熊超,段冲,徐俊超,何媛媛,张悦,曾林涛..比率型SO2荧光探针的构建及细胞成像研究[J].影像科学与光化学,2016,34(5):435-443,9.基金项目
国家自然科学基金(21203138)和天津理工大学国家级大学生创新训练项目(201510060009)资助 （21203138）
