临床与病理杂志2017，Vol.37Issue(1)：29-34,6.DOI:10.3978/j.issn.2095-6959.2017.01.006
过表达细胞黏附分子1抑制卵巢癌细胞迁移及侵袭
Overexpression of cell adhesion molecule 1 inhibits the migration and invasion of ovarian carcinoma
王晓凤 1柳艳飞 1田勇 1何为 1金红艳1
作者信息
- 1. 武汉科技大学附属武汉市普仁医院肿瘤科,武汉430000
- 折叠
摘要
Abstract
Objective:To investigate the effect of over expression of cell adhesion molecule 1 (CADM 1) on cell proliferation,migration and invasion of ovarian carcinoma.Methods:The expression level of CADM1 mRNA of SKOV3 and hose was measured by qRT-PCR.The SKOV3 cell line was divided into two groups,CADM1 over-expression group was transfected with pcDNA3.1-CADM1 plasmid,and control group with pcDNA3.1 plasmid by lipofectamine 2000.The expression level of CADM1 of those two groups was measured by Western Blot.The proliferation,clone formation,migration and invasive ability was measured and compared between those two groups by CCK-8,clone formation,cell scratch-wound and Transwell assay respectively.Results:The expression level of CADM l mRNA was 1.54±0.34 in SKOV3 cell line and 5.63±0.96 in hose normal cell line,the difference was statistically significant (P＜0.05).The protein expression level in CADM1 over-expression group and control group was 2.53±0.42 and 0.37±0.09 respectively after transfected for 48 h,the difference was statistically significant (P＜0.05).There was no significant difference between those two groups in terms of OD value of 450 nm in 0,24,48 and 72 h (P＞0.05).The number of clone cells was 60.4±7.6 in CADMl over-expression group,which was higher than that (58.3±8.2) in control group,the difference has no statistical significance (P＞0.05).The cell migration rate of CADM1 over-expression group was significantly lower than control group (20.3％±3.5％ vs.60.1％±4.2％,P＜0.05).The invasive cell number of CADM1 over-expression group was significantly lower than control group (24.5±5.3 vs.65.1±6.9,P＜0.05).Conclusion:CADM1 showed low expression in ovarian carcinoma cell line.Over-expression of CADM 1 had no influence on proliferation and clone formation in ovarian carcinoma cell line,while it inhibits the migration and invasive ability which demonstrates that CADM1 was a tumor suppressor.关键词
细胞黏附分子1/卵巢癌/迁移/侵袭Key words
cell adhesion molecule 1 (CADM1)/ovarian carcinoma/migration/invasion引用本文复制引用
王晓凤,柳艳飞,田勇,何为,金红艳..过表达细胞黏附分子1抑制卵巢癌细胞迁移及侵袭[J].临床与病理杂志,2017,37(1):29-34,6.
