摘要
Abstract
Objective:To investigate the immune phenotype characteristics of tumor-associated fibroblasts (TAF) of malignant mesothelioma in the diagnosis and treatment by observing its immune phenotype.Methods:We respectively collected 10 cases of malignant mesothelioma,esophageal cancer,breast cancer,and colon cancer specimens,the expression of CK,CK19,Calretinin,HBME-1,Vimentin,Sny,S-100 of the TAF was observed in these tumors.Results:The TAF in 10 cases of malignant mesothelioma,esophageal squamous cell carcinoma,breast invasive ductal carcinoma,and colon adenocarcinoma were positive for Vimentin,and positive for Syn,S-100 in partial region.The TAF in 10 cases of malignant mesothelioma were positive for CK,CK19,Calretinin,and weak positive for HBME-1;10 cases of esophageal squamous cell carcinoma,breast invasive ductal carcinoma,and colon adenocarcinoma were weak positive for CK and Calretinin in partial region,and negative for CK19 and HBME-1.The integrated optical density (IOD) value of TAF expressing Calretinin,CK19,CK and HBME-1 in malignant mesothelioma group were significantly higher than those in the other three groups (P＜0.05).Conclusion:The TAF of malignant mesothelioma are both common and different from the TAF in common tumor.It is of great significance to study the diagnosis and treatment of malignant mesothelioma by studying the immune phenotype and molecular biological characteristics of the TAF.关键词
恶性间皮瘤/成纤维细胞/肿瘤/间质/免疫组织化学Key words
malignant mesothelioma/fibroblasts/tumor/stroma/immunohistochemistry
