临床与病理杂志2017，Vol.37Issue(1)：133-136,4.DOI:10.3978/j.issn.2095-6959.2017.01.023
Fibulin-1基因启动子区域甲基化与食管癌的关系研究
Relationship of promoter region methylation of Fibulin-1 gene and esophageal cancer
王静 1赵亮1
作者信息
- 1. 鄂东医疗集团黄石市中心医院,湖北理工学院附属医院普爱院区消化内科,湖北黄石435000
- 折叠
摘要
Abstract
Objective:To detect promoter region methylation of Fibulin-1 gene in esophageal cancer and explore its clinical significance.Methods:Ninety-six patients of esophageal cancer admitted from January 2014 to February 2016 in Huangshi Central Hospital were enrolled in this study.Another 40 cases of benign esophageal tissues were used as control.Fibulin-1 gene promoter was modified by bisulfite and methylation was detected using PCR analysis.Fibulin-1 protein expression was detected by immunohistochemistry.Results:The positive rate of Fibulin-1 gene promoter methylation in patients with esophageal cancer was 36.5％,which was significantly higher than that of 15.0％ in control group (x2=2.517,P=0.036).The positive expression rate of Fibulin-1 protein in the promoter methylation ofFibulin-1 gene was 90.2％.The positive rate ofFibulin-1 gene promoter methylation has no significant difference in different gender and age in bladder cancer (P＞0.05).The positive rate of Fibulin-1 gene promoter methylation in Ⅲ and Ⅳ stage group was 43.1％,which was higher than that of 28.9％ in Ⅰ and Ⅱ stage group (x2=2.426,P=0.046).The positive rate ofFibulin-1 gene promoter methylation in lymph node metastasis group was 44.4％,which was higher than that of 26.2％ in non-lymph node metastasis group (x2=2.438,P=0.041).The positive rate ofFibulin-1 gene promoter methylation in median and low differentiation group was 42.6％,which was higher than that of 25.7％ in well-differentiation group (x2=2.431,P=0.043).Conclusion:Hypermethylation ofFibulin-1 gene was detected in esophageal cancer,which was related to clinical stages,lymph node metastasis,and tumor differentiation of esophageal cancer.关键词
食管癌/Fibulin-1基因/甲基化Key words
esophageal cancer/Fibulin-1 gene/methylation引用本文复制引用
王静,赵亮..Fibulin-1基因启动子区域甲基化与食管癌的关系研究[J].临床与病理杂志,2017,37(1):133-136,4.
