临床与病理杂志2017，Vol.37Issue(3)：473-477,5.DOI:10.3978/j.issn.2095-6959.2017.03.005
TaqMan探针法与尿素呼气试验法检测胃镜活检标本幽门螺旋杆菌的比较
Comparison of TaqMan PCR method and Carbon-14-Urea breath test in the diagnosis of Helicobacter pylorus
摘要
Abstract
Objective: To investigate and compare TaqMan PCR method and Carbon-14-Urea breath test on Helicobacter pylori (Hp) detection. Methods: TaqMan PCR method was used in 70 cases of clinical samples, and the results were analyzed. Results: There was no difference in the positive rate of Hp in TaqMan PCR method and Carbon-14-Urea breath test, and there was a good correlation between the two methods, but the positive rate was higher in Carbon-14-Urea breath test. Further study showed that the positive intensity of Hp can be divided into 3 types by TaqMan PCR method, and there was a good correlation between positive intensity and pathological degree of inflammation. Conclusion: TaqMan PCR method can be used as a useful molecular biological method for clinical diagnosis of Hp.关键词
幽门螺旋杆菌/TaqMan探针法/尿素呼气试验法Key words
Helicobacter pylori/TaqMan PCR method/Carbon-14-Urea breath test引用本文复制引用
沈和德,褚明亮,易韦,杨文秀..TaqMan探针法与尿素呼气试验法检测胃镜活检标本幽门螺旋杆菌的比较[J].临床与病理杂志,2017,37(3):473-477,5.基金项目
国家自然科学基金(81560088).This work was supported by National Natural Science Foundation of China (81560088). （81560088）
