临床与病理杂志2017，Vol.37Issue(3)：570-575,6.DOI:10.3978/j.issn.2095-6959.2017.03.021
一氧化氮对鼻咽癌CNE-2细胞增殖与凋亡的影响
Effect of proliferation and apoptosis induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cell
摘要
Abstract
Objective: To investigate the effect of proliferation and apoptosis induced by exogenous NO on CNE-2 cells. Methods: CNE-2 cells were treated by different concentrations of sodium nitroprusside (SNP). The morphological changes of cells were observed, the viability and apoptosis of cells were examined. Results: SNP inhibited proliferation of CNE-2 cell in a concentration-dependent manner, CNE-2 cell were treated by SNP 100, 200, 400, 600, 800, 1600, 3200 μmol/L group respectively and the inhibition rate of the cells of each group was positively correlated with the concentration of SNP. SNP promote apoptosis of CNE-2 cell in a concentration-dependent manner; compared with SNP 600 μmol/L, flow cytometry analysis showed that the apoptosis rate of CNE-2 cells on the group treated by SNP 1000 μmol/L increased significantly (P<0.05). Conclusion: Exogenous nitric oxide can inhibit CNE-2 proliferation and promote apoptosis. The inhibitory effect was positively correlated with the concentration of NO.关键词
一氧化氮/硝普钠/鼻咽癌/凋亡Key words
NO/sodium nitroprusside/nasopharyngeal carcinoma/apoptosis.引用本文复制引用
张彩霞,刘阳云,刘庚勋,江文,曹杭,陈琼,常恩格,张纪帅..一氧化氮对鼻咽癌CNE-2细胞增殖与凋亡的影响[J].临床与病理杂志,2017,37(3):570-575,6.基金项目
湖南省研究生科研创新项目(CX2016B225) （CX2016B225）
湖南省教育厅科学研究项目(14C0697).This work was supported by the Hunan Provincial Graduate Student Research Innovation Project (CX2016B225) （14C0697）
Hunan Provincial Education Department Science Research Project, P. R. China (14C0697). （14C0697）
