光学精密工程2017，Vol.25Issue(3)：562-568,7.DOI:10.3788/OPE.20172503.0562
降钙素原胶体金试纸条显色分析仪
Color analytical reader for procalcitonin colloidal gold test strips
摘要
Abstract
The semi-quantitative method of Colloidal gold strip detection by colorimetric principle is widely used in biomedical diagnosis.To achieve quantitative measurement of calcitonin concentration,a CMOS color analytical reader based on raspberry Pi was designed for the acquisition of color characteristics of chromogenic reaction by taking a picture of colloidal gold strip.According to the color characteristics of procalcitonin colloidal gold test strips,the CMOS sensor was corrected by proper white balance parameter adjustment,which enabled the CMOS image sensor has a better color restoration property.In order to eliminate the difference of color management between different devices,the mathematical model of the sensor from RGB space to XYZ space was established via polynomial regression by taking pictures of NCS standard color cards.Thus the instrument was adjusted in color characteristics.Then,the mathematical model of XYZ values and concentration values of the samples was derived from the representative gray value pixel areas of the processed images,thereby the color uniformity problem was solved and the concentration was accurately measured.The experiment results show that the measuring precision can be as high as 93.8％,the repeated measurement error is less than 1.5 ％ and the effective measurement range is from 0.1 ng/mL to 35 ng/mL.With the virtues of cost effective and high measurement speed,the analyzer has a promising application prospect.关键词
色度分析/胶体金/颜色空间/多项式回归/降钙素/浓度测量Key words
chrominance analysis/colloidal gold/color space/polynomial regression/procalcitonin/concentrate measurement分类
机械制造引用本文复制引用
王霞,陈飞,宿宸铭,黎先龙..降钙素原胶体金试纸条显色分析仪[J].光学精密工程,2017,25(3):562-568,7.基金项目
博士点基金资助项目(No.20131101130002) （No.20131101130002）
