广西科学2017，Vol.24Issue(1)：73-76,4.DOI:10.13656/j.cnki.gxkx.20170223.002
丝状真菌启动子的基因工程改造进展
Research Progress Towards Promoters Genetic Engineering in Filamentous Fungi
摘要
Abstract
Filamentous fungi are important industrial microbes with excellent ability of protein expression and secretion.For example,Trichoderma reesei can produce its endogenouscellulase with a titer of up to 100 g/L in industrial fermentation conditions.Currently,filamentous fungi have been successfully used to express heterologous genes.However,compared with endogenous gene expression,the expression of heterologous genes in filamentous fungi is often of low efficiency.In order to increase the expression level of heterologous genes,one effective way is to use strong promoters to drive gene transcription.In this paper,we discuss the progress of genetic modification of the filamentous fungi promoters to improve transcription efficiency of endogenous and heterologous genes.关键词
丝状真菌/启动子/基因工程Key words
filamentous fungi/promoter/genetic engineering分类
生物科学引用本文复制引用
孙先花,苏小运..丝状真菌启动子的基因工程改造进展[J].广西科学,2017,24(1):73-76,4.基金项目
国家自然科学基金青年基金项目"多基因协同调控里氏木霉高效分泌表达蛋白的研究"(项目批准号:31400067)资助. （项目批准号:31400067）
