广西科学2017，Vol.24Issue(1)：112-119,8.DOI:10.13656/j.cnki.gxkx.20170124.001
里氏木霉和黑曲霉产酸性木聚糖酶及酶学特性比较
Comparative Analysis of the Characterization of Crude Acidic Xylanases from two Strains between Trichoderma reesei and Aspergillus niger
摘要
Abstract
[Objective]To identify potential ideal acidic xylanase, the characterizations of crude acidic xylanase from two strains between Trichoderma reesei and Aspergillus niger are analyzed and compared,especially when the xylanase activity and stability are determined at pH 4.[Methods]These two strains mentioned above are inoculated into culture medium for xylanase production,and the enzyme activity and characterizations of crude acid xylanase are analyzed.[Results]The activities of acidic xylanase and xylosidase from Aspergillus niger are up to (52.36±2.61)U/mL and (0.57±0.01)U/mL respectively,and the optimum temperature and pH value of acidic xylanase from this strain are 55℃and 5.0,while the optimum temperature and pH value of acidic xylosidase from this strain are 75℃and 5.0,respectively.With constract to Trichoderma reesei,they are (10.12±0.95)U/mL and (0.32±0.05)U/mL,65℃and 6.5,65℃ and 4.5,correspondingly.In addition, both of the acidic xylanases produced by Aspergillus niger and Trichoderma reesei have acidic CMCase activity,of which Aspergillus niger is (5.26±0.21) U/mL and Trichoderma reesei is (1.72±0.21) U/mL,respectively.[Conclusion]The acidic xylanase produced by Aspergillus niger is potential ideal acidic xylanase,which is better than the one produced by Trichoderma reesei.关键词
黑曲霉/里氏木霉/酸性木聚糖酶/酶学特性Key words
Aspergillus niger/Trichoderma reesei/acidic xylanase/enzyme properties分类
生物科学引用本文复制引用
吴仁智,黄俊,芦志龙,陈英,陆琦,陈小玲,陈东,黄日波..里氏木霉和黑曲霉产酸性木聚糖酶及酶学特性比较[J].广西科学,2017,24(1):112-119,8.基金项目
国家"863"项目(2013AA050701),国家国际合作项目(2011DFA61910),国家科技计划课题子任务(2012AA022106),广西科技合作与交流计划(桂科合2015DD23055),广西重点研发计划(桂科AB16380024),广西科技攻关/主席科技资金(1517-07),广西科学院基本科研业务费项目(12YJ25SW04),八桂学者建设工程专项经费项目,广西生物质产业化工程院建设(桂科能12237022)和广西生物炼制重点实验室项目(13-051-08)资助. （2013AA050701）
