南方农业学报2017，Vol.48Issue(1)：92-99,8.DOI:10.3969/j:issn.2095-1191.2017.01.92
荷花腐败病生防菌的筛选及鉴定
Screening and identification of biocontrol bacterium against lotus rhizome rot disease
摘要
Abstract
[Objective]The present study screened and identified the biocontrol bacteria against Fusarium commune,in order to provide reference for healthy planting of lotus and effective control of lotus rhizome rot disease.[Method] Inhibition zone method and confrontation culture were employed to screen the biocontrol bacteria which had strong antagonism against F.commune out of 106 tested antagonistic bacteria.The biocontrol bacteria were identified according to morphology,physiology,biochemistry and molecular identification.Through confrontation culture with six phytopathogenic fungi,antimicrobial spectrum of the biocontrol bacteria was studied.Field control effects of biocontrol bacterium were explored through field experiment.[Result]Four biocontrol bacteria with antagonism against lotus rhizome rot disease were selected out of 106 tested strains.Antagonist effects of strain DR1 was the best.Its bacteriostasis band width was 9.8 mm.Strain DR1 enjoyed wide antimicrobial spectrum,and showed inhibitory effects towards six tested phytopathogenic fungi.The field experiment results showed that average control effect of strain DR 1 was 41.83％.Strain DR1 was identified as Bacillus amyloliquefaciens for its biochemical characteristics based on 16S rDNA and gyrB gene sequence analysis.[Conclusion]B.amyloliquefaciens has strong antagonism effect,heat resistance,strong stress resistance,and broad antimicrobial spectrum.Therefore,it possesses promising developing and applying prospect in lotus disease control.关键词
荷花腐败病/生物防治/解淀粉芽孢杆菌/鉴定Key words
lotus rhizome rot disease/biological control/Bacillus amyloliquefaciens/identification分类
农业科技引用本文复制引用
曾莉莎,王芳,庄华才,胡规媛,吕顺,莫秀文,杜彩娴,梁少丽,郑芝波,周建坤..荷花腐败病生防菌的筛选及鉴定[J].南方农业学报,2017,48(1):92-99,8.基金项目
广东省科技计划项目(2015A020209002) （2015A020209002）
东莞市社会科技发展项目(2014108101044) （2014108101044）
