南方农业学报2017，Vol.48Issue(1)：109-113,5.DOI:10.3969/j:issn.2095-1191.2017.01.109
金花葵多糖提取的工艺优化及抗氧化活性研究
Optimizing extraction technique of Hibiseu Smanihot L.polysaccharides and its antioxidant activity
摘要
Abstract
[Objective]The extraction process of polysaccharides from Hibiseu smanihot L.was optimized,and its antioxidant activity was investigated,in order to provide basis for research and utilization of polysaccharides from H.smanihot L.[Method]Taking extraction rate of H.smanihot L.polysaccharides as index,and on the basis of single factor experiment,the extraction technology assisted by ultrasonic was optimized by L9(33) orthogonal test.The antioxidant activities of H.smanihot L.polysaccharides were evaluated through scavenging experiment on DPPH free radical and the hydroxyl free radical (· OH).[Result]The three factors affecting ultrasonic-assisted extraction of H.Smanihot L.polysaccharides were in order as follows:ultrasonic time＞material-liquid ratio＞ultrasonic temperature.The optimum extraction process parameters of polysaccharides were as follows material-liquid ratio 1∶50,ultrasonic time of 30 min,ultrasonic temperature 50 ℃.Under such condition,the extraction rate reached 22.32％.Free radical scavenging experiment showed that scavenging effects of H.Smanihot L.polysaccharides towards DPPH free radical and · OH varied according to the dosage.[Conclusion]The optimized extraction process of H.Smanihot L.polysaccharides is simple,convenient and feasible.And H.Smanihot L.polysaccharides has strong antioxidant activity.This processing technique can be applied in extraction and development of H.Smanihot L.polysaccharides.关键词
金花葵/多糖/超声提取/抗氧化活性Key words
Hibiseu smanihot L./polysaccharides/ultrasonic extraction/antioxidant activity分类
化学化工引用本文复制引用
巫玲丽,穆祯强,张利..金花葵多糖提取的工艺优化及抗氧化活性研究[J].南方农业学报,2017,48(1):109-113,5.基金项目
四川理工学院人才引进项目(2015RC24) （2015RC24）
