南方农业学报2017，Vol.48Issue(1)：119-126,8.DOI:10.3969/j:issn.2095-1191.2017.01.119
马氏珠母贝肉酶解条件优化及其产物活性分析
Optimization of enzymolysis conditions for Pinctada fuctada martensii muscle and activity analysis of enzymatic hydrolysate
摘要
Abstract
[Objective]The present study optimized ultrasonic-assisted enzymolysis conditions for Pinctada fuctada martensii muscle,and analyzed free radical scavenging activity of enzymatic hydrolysate,in order to provide reference for value-added utilization of P.fuctada martensii muscle.[Method] P.fuctada martensii muscle was taken as the material,single factor and response surface methodology were applied to analyze effects of enzymolysis temperature,protease dosage,ultrasonic power and enzymolysis time on degree of hydrolysis(DH) of protein in P.fuctada martensii muscle,and determine the optimum process parameters,and measure scavenging activity of enzymatic hydrolysate on hydroxyl radical (· OH),superoxide radical(O2) and DPPH free radical(DPPH·).[Result]The factors influencing enzymolysis of P.fuctada martensii muscle in order of their effects were as follows:ultrasonic power＞protease dosage＞enzymolysis temperature＞enzymolysis time.Enzymolysis temperature,protease dosage and ultrasonic power had extremely significant difference on enzymolysis(P＜0.01),and interaction between ultrasonic power and enzymolysis time had significant difference (P＜0.05).The optimal enzymolysis condition was as follows:enzymolysis temperature was 48 ℃,protease dosage 2200 U/g,ultrasonic power 13％ (total output power was 900 W),enzymolysis time 12 min.Under such condition,DH of protein was 21.937％,the relative error to expected value(22.140％) was 0.92％.The scavenging activity of enzymatic hydrolysate on · OH,O~ and DPPH· enhanced as mass concentration of enzymatic hydrolysate increased.When the mass concentration of enzymatic hydrolysate reached 2.0 mg/mL,the scavenging rate for ·OH was 68.85％,scavenging rate for O2-83.62％ and scavenging rate for DPPH· 82.36％.[Conclusion]The optimized process parameters of ultrasound-assisted enzymolysis are accurate and reliable.Enzymolysis time is shortened and free radical scavenging activity of enzymatic hydrolysate is obvious.Therefore,the enzymatic hydrolysate can be utilized as raw materials of health care food,health care drinks with antioxidation.关键词
马氏珠母贝肉/超声波辅助酶解/响应面法/清除自由基活性Key words
Pinctada fuctada martensii muscle/ultrasonic-assisted enzymolysis/response surface methodology/free radicals scavenging activity分类
轻工纺织引用本文复制引用
付晶晶,张艳雯,吴耀生,孙健..马氏珠母贝肉酶解条件优化及其产物活性分析[J].南方农业学报,2017,48(1):119-126,8.基金项目
广西高校科学技术研究项目(KY2015YB458) （KY2015YB458）
