南方农业学报2017，Vol.48Issue(3)：441-447,7.DOI:10.3969/j:issn.2095-1191.2017.03.010
瓠瓜砧木嫁接西瓜苗对枯萎病的抗性综合评价
Comprehensive resistance evaluation of grafting watermelon seedlings on different bottle gourd stocks to watermelon fusarium wilt
摘要
Abstract
[Objective]In the present study, resistance of grafting watermelon seedlings on different bottle gourd stocks to watermelon fusarium wilt and the differences in growth and physiological indexes were analyzed, key indexes for evaluat-ing disease resistance were established, in order to offer reference for selecting bottle gourd stocks and resistant variety breeding. [Method]Grafting watermelon seedlings on six different bottle gourd stock types(H04,H05,H07,H10,H0405 and H1004) were taken as materials, and self-grafted watermelon seedlings were used as control. Disease resistance of grafting watermelon seedlings on bottle gourd stock was identified through inoculation of Fusariumoxysporum f. sp. niveum on graft-ing seedlings. Plant height, stem diameter, maximum leaf area, leaf chlorophyll content(SPAD value), leaf peroxidase (POD) activity, and leaf malondialdehyde(MDA) content of grafting watermelon seedlings were determined. Resistance of different grafting seedlings to watermelon fusarium wilt was evaluated by subordinate function. [Result]Different grafting seedlings presented different resistance performance to watermelon fusarium wilt. The seedlings grafting on H0405 and H04 showed high resistance. These seedlings had the largest maximum leaf area and highest SPAD value. The growth of plant height and stem diameter was fast. Leaf POD activity of the seedlings was significantly higher than that of self-grafted wa-termelon seedlings yet MDA content was significantly lower than that of self-grafted watermelon seedlings(P<0.05). Sub-ordinate function based on growth and physiological indexes was adopted to comprehensively evaluate disease resistance of grafting watermelon seedlings on different bottle gourd stocks. The results were in accordance with the results based on rank of plant height, maximum leaf area, SPAD value and disease index. [Conclusion]High generation inbred line H04 can be breed-ing resource of bottle gourd as stocks with high resistance to watermelon fusarium wilt. Its first generation of hybrid H0405 can be good stock for watermelon with high disease resistance to watermelon fusarium wilt. Height, maximum leaf area and leaf chlorophyll content(SPAD value) of grafting seedlings can be useful indexes to evaluate resistance to watermelon fusarium wilt.关键词
西瓜枯萎病/砧用瓠瓜/嫁接/抗病性/综合评价Key words
watermelon fusarium wilt/bottle gourd stock/grafting/disease resistance/comprehensive evaluation分类
农业科技引用本文复制引用
鲍婉雪,钟川,赵文宗,廖建杰,陈文明,凌志阳,阳燕娟,于文进..瓠瓜砧木嫁接西瓜苗对枯萎病的抗性综合评价[J].南方农业学报,2017,48(3):441-447,7.基金项目
国家自然科学基金项目(31401858) （31401858）
广西科学研究与技术开发计划项目(桂科合1347004-10,桂科合14123001-11) （桂科合1347004-10,桂科合14123001-11）
广西自然科学基金项目(2014GXNSFBA118082) （2014GXNSFBA118082）
