广西植物2017，Vol.37Issue(3)：308-314,7.DOI:10.11931/guihaia.gxzw201604019
红海榄内生细菌多样性及其抑制鱼类致病菌活性研究
Diversity of cultivated marine bacteria and antibacterial activity of endophytic bacterial in Rhizophora stylosa
摘要
Abstract
Rhizophora stylosa is an evergreen tree with prop roots, and a common tree found in tropical coastal mangrove forests, widely distributed in tropical coastal mudflats.As a typical representative of the mangrove, R.stylosa has rich endophytic diversity with little research.The study objects of R.stylosa were collected from Shankou National Nature Reserve, Guangxi, China.From stem, root, leaf and other tissues, seventeen endophytes were isolated with the dilution coating method and the three-wire method.We Observed their morphological characteristics, 16S rRNA and Venny Chart were utilized to analyze the diversity of their endogenous bacterial.Depending on the results of diversity analysis, we could find that these strains have different morphological characteristics and they could classified into three doors, seven genera, five families and eight species.Further analysis showed that endophytic bacteria groups of R.stylosa root, stem, leaf and other organizations vary different, only one same genus was Mangrovibacter plantisponsor.Micromonospora and Mangrovibacter genus were its dominant bacteria, the proportion was 29.4% and 17.6%, respectively.Among them, the most endophytic bacteria within the root, the proportion was 47.1%.Seventeen strains belong to three doors, wherein the most number of bacteria were Proteobacteria, accounting for 52.9%, then Actinomycetes door accounted for 41.2%.In those isolated strains, three strains of bacterial species with the full-length 16S rRNA gene similarity less than 97%, may be possible new genera or new genus and had potential research value.Vibrio parahaemolyticus was a zoonotic bacteria caused some damage to uman health, severe cases even death.To test anti Vibrio parahaemolyticus activity of the crude extract of strains isolated, we used filter paper method, and the results indicated that three strains(H003, H009, H013, 5 mg·mL-1) had a strong antibacterial activity against Vibrio parahaemolyticus, their inhibition zone could reach (8.4±0.07), (8.2±0.07), and (8.3±0.14) mm, respectively.Endophytic bacteria of Rhizophora stylosa were genetically diverse and most of them showed strong inhibition effect against Vibrio parahaemolyticus.The results provides the essential material basis for the further study of its chemical diversity and utilization of resources.关键词
红海榄/内生细菌/物种多样性/副溶血弧菌/抑菌活性Key words
Rhizophora stylosa/endophytic bacterial/diversity/Vibrio parahaemolyticus/antibacterial activity分类
农业科技引用本文复制引用
李家怡,周文红,李菲,高程海,易湘茜..红海榄内生细菌多样性及其抑制鱼类致病菌活性研究[J].广西植物,2017,37(3):308-314,7.基金项目
广西自然科学基金(2015GXNSFBA139195，2015GXNSFBB139001)； 广西高校科学技术研究项目(KY2015YB146)； 广西科学研究与技术开发计划项目(桂科合15104001-11)； 广西中药药效研究重点实验室项目(16-380-29)[Supported by Guangxi Natural Science Foundation (2015GXNSFBA139195，2015GXNSFBB139001)； Guangxi University of Science and Technology Research Program(KY2015YB146)； Key Program of Science and Technology Development Program of Guangxi (15104001-11)； Guangxi Key Laboratory of Generic Technology Research and Development of TCM Preparation(16-380-29)]。 （2015GXNSFBA139195，2015GXNSFBB139001）
