广西植物2017，Vol.37Issue(3)：329-334,6.DOI:10.11931/guihaia.gxzw201609020
基于ITS序列分析钩苞大丁草九个居群的亲缘关系
Genetic relationship analysis of Gerbera delavayi from nine populations based on ITS sequences
摘要
Abstract
Ribosomal DNA (rDNA) ITS of nine populations of Gerbera delavayi in Southwest China were amplified, sequenced and compared with G.jamesonii.The relationships of geographic distance and genetic distance of nine populations were studied.The NJ phylogenetic tree was also constructed.The results were as follows: (1) The whole length of ITS sequences of G.delavayi was 600-700 bp, the average length was 657 bp, the length of ITS1 was 243-246 bp, (G+C) amounted for 45.67%-46.80%, the length of 5.8S was 191-193 bp , (G+C) amounted for 58.60%-58.61%, the length of ITS2 was 220-221 bp, (G+C) amounted for 57.00%-57.45%.A total of 22 variable sites (ITS1, 7;5.8S, 2 and ITS2, 3) were found.(2) The genetic distance had positive correlation with the geographic distance of G.delavayi (r2=0.652).The genetic differentiation distance was 0.001 1-0.024 3, among which the genetic distance between Pu'er and other population was the largest.(3) Nine populations were divided into three branches, in which Pu'er populations was separated as the first group.The second group contained Lijiang and Eryuan provenances.The other six populations (Fuyuan, Wuding, Dechang, Shilin, Xinping and Kaiyuan) were clustered together.RDNA ITS sequence analysis could be applied to study the population genetic of G.delavayi.The results provides reference for the protective development of G.delavayi.关键词
钩苞大丁草/ITS序列/居群/遗传距离/亲缘关系Key words
Gerbera delavayi/ITS sequences/population/genetic distance/genetic relationship分类
农业科技引用本文复制引用
王谈笑,郑伟,陈菁,王炜,徐晓丹..基于ITS序列分析钩苞大丁草九个居群的亲缘关系[J].广西植物,2017,37(3):329-334,6.基金项目
国家自然科学基金(31560086) [Supported by the National Natural Science Foundation of China(31560086)]. （31560086）
