解放军医药杂志2017，Vol.29Issue(1)：10-14,5.DOI:10.3969/j.issn.2095-140X.2017.01.003
高糖对原代人脐静脉内皮细胞的损伤作用及机制研究
Injury Effect of High Glucose on Original Generation of HUVECs and Its Mechanism
摘要
Abstract
Objective To investigate injury effect of high glucose concentration on original generation of human umbilical vein endothelial cells (HUVECs) and its injury mechanism.Methods HUVECs were cultured by medium of low concentration of glucose (LG,5.5 mmol/L) and high concentration of glucose (HG,35 mmoL/L) respectively for 24 h,72 h and 120 h.Effect of HG on survival rate of HUVECs was detected by using cholecystokinin-8 (CCK-8) assay,and reactive oxygen species (ROS) and nitric oxide (NO) levels were detected using fluorescent probe,and then expressions of HG on Bax,Bcl-2,silent mating type information regulation 2 homolog 1 (SIRT1) and endothelial nitric oxide synthase (eNOS) expressions were detected by western bolt method.Results In HG group,after culturing for 72 h and 120 h,values of survival rate and Bcl-2 were lower,while values of ROS and Bax/Bcl-2 ratio were higher than those in LG group (P ＜ 0.05);after culturing for 24 h,72 h and 120 h,relative amounts of SIRT1,and eNOS were lower than those in LG group;after culturing for 72 h and 120 h,eNOS expressions were lower than those in LG group (P ＜ 0.05).Conclusion HG may decrease survival rate,increase of intracellular oxidative stress reaction,improve Bax/Bcl-2 ratio of HUVECs,which may be associated with inhibition of SIRT1-eNOS pathway to reduce NO production.关键词
人脐静脉内皮细胞/一氧化氮/高糖/沉默信息调节因子1Key words
Human umbilical vein endothelial cell/NO/High glucose/Silent mating type information regulation 2 homolog 1分类
医药卫生引用本文复制引用
哈小琴,王娟,白燕青,徐倩,曹荟哲,曾通旭,杨志华..高糖对原代人脐静脉内皮细胞的损伤作用及机制研究[J].解放军医药杂志,2017,29(1):10-14,5.基金项目
国家自然科学基金(81273568) （81273568）
