华北农学报2016，Vol.31Issue(6)：83-87,5.DOI:10.7668/hbnxb.2016.06.013
基于PCR方法对载体pBluescriptⅡSK(+)及pCAMBⅠA1300的定点突变
Site-directed Mutagenesis of pBluescriptⅡSK (+) and pCAMBⅠA1300 Vectors Based on PCR Method
摘要
Abstract
In order to provide more convenient and efficient restriction enzyme site for the construction of CRISPR/Cas9 gene editing system in the future,the multiple clone site of pBluescript Ⅱ SK(+) and pCAMB Ⅰ A1300 vectors were modified.DNA sequences showed that site-directed mutagenesis were successfully implemented in both pBluescript Ⅱ SK(+) and pCAMB Ⅰ A1300 vectors.The four clone sites of pBluescript Ⅱ SK(+) about Xho Ⅰ,EcoR V,Sma Ⅰ,Sac Ⅱ were transformed to Nhe Ⅰ,Mfe Ⅰ,Nsi Ⅰ,Pac Ⅰ respectely;Two clone sites of pCAMB Ⅰ A1300,Sac Ⅰ and Sal Ⅰ were changed to Nsi Ⅰ,Pac Ⅰ.Thus,lay solid foundation for further use on CRISPR/Cas9 genome editing vector.关键词
pBluescriptⅡSK(+)/pCAMBⅠA1300/定点突变/PCRKey words
pBluescript Ⅱ SK(+)/pCAMB Ⅰ A1300/Site-directed mutagenesis/PCR分类
生物科学引用本文复制引用
蒲艳,刘超,林琪,李继洋,刘晓东..基于PCR方法对载体pBluescriptⅡSK(+)及pCAMBⅠA1300的定点突变[J].华北农学报,2016,31(6):83-87,5.基金项目
国家自然科学基金项目(31560534) （31560534）
