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纳米处理对小麦幼胚组织培养效果及其S1变异的影响

孙岩 张宏纪 刘东军 刘文林 王广金 杨淑萍 林婷婷 赵远玲

黑龙江农业科学Issue(2):18-22,5.
黑龙江农业科学Issue(2):18-22,5.DOI:10.11942/j.issn1002-2767.2017.02.0018

纳米处理对小麦幼胚组织培养效果及其S1变异的影响

Effect of Nano Treatment on the Effect of Tissue Culture on Wheat Young Embryo and Mutation of S1 Generation

孙岩 1张宏纪 1刘东军 1刘文林 1王广金 2杨淑萍 1林婷婷 1赵远玲3

作者信息

  • 1. 黑龙江省农业科学院 作物育种研究所,黑龙江 哈尔滨 150086
  • 2. 黑龙江省农业科学院 大豆研究所,黑龙江 哈尔滨 150086
  • 3. 黑龙江省农业科学院生物技术研究所
  • 折叠

摘要

Abstract

In order to probe into the effect of nano treatment on effect of tissue culture on wheat young embryo and mutation of S1 generation,explant medium in use nano treated purified water had been compounded,and comparison explant medium compounded by common purified water from 2013 to 2015.Frequence of induction,frequence of regeneration and mutation of important agricultural characters for S1 generation were investigated.The results showed that mean frequence of regeneration on treatmented explant medium for materials Long0632 and 93-6529 was 9.3%,and their mean frequence was 5.4% as control.The mean variable coefficient of plant hight,the mean variable coefficient of spike longth and the rate of select on treatmented explant medium for materials Long0632 and 93-6529 were 4.7%,2.1% and 7.1% respectively,and their mean variable coefficient of plant hight,mean variable coefficient of spike longth and rate of select were 0.4%,1.0% and 2.7% respectively as control.The nano treatmentcould enhance effect of tissue culture for wheat young mebryo and mutations of progeny,also can help breeding of wheat somatic mutation.

关键词

小麦/纳米处理/组织培养/体细胞变异

Key words

wheat/nano treatment/tissue culture/somatic mutation

分类

农业科技

引用本文复制引用

孙岩,张宏纪,刘东军,刘文林,王广金,杨淑萍,林婷婷,赵远玲..纳米处理对小麦幼胚组织培养效果及其S1变异的影响[J].黑龙江农业科学,2017,(2):18-22,5.

基金项目

哈尔滨市科技局科技创新人才研究专项资金资助项目(2016RAQXJ212) (2016RAQXJ212)

国家重点研发计划资助项目(2016YFD0102101) (2016YFD0102101)

黑龙江农业科学

1002-2767

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