河南农业科学2017，Vol.46Issue(1)：13-18,25,7.DOI:10.15933/j.cnki.1004-3268.2017.01.003
郑麦366α-醇溶蛋白基因的克隆及生物信息学分析
Cloning and Bioinformatic Analysis of α-gliadin Genes in Zhengmai 366
摘要
Abstract
To reveal the composition of α-gliadin protein in high quality and strong gluten wheat variety Zhengmai 366,the PCR amplification were applied to clone α-gliadin genes in Zhengmai 366 with degenerate primers.In total,13 nucleotide sequences were amplified,while the deduced amino acid sequences of nine encoded complete open reading frames.Further analysis showed that the cloned genes (named as ZM366-1-ZM366-9 respectively) had the typical characteristics of α-gliadin.According to the number of T cell stimulatory toxic epitopes and the characteristics of polyglutamine domain,ZM366-1 and ZM366-2,ZM366-3 and ZM366-4 and ZM366-5-ZM366-9 were localized to the 6A,6D and 6B chromosome respectively.Secondary structure prediction showed that all the nine α-gliadin cloned in the present study only contained α-helix structure,and the content of α-helix was significantly higher in α-gliadin of B genome than that of others.Phylogenetic tree analysis showed that the cloned α-gliadin of present study had obvious genomic specificity.关键词
小麦/α-醇溶蛋白/克隆/生物信息学Key words
wheat/α-gliadin/cloning/bioinformatic analysis分类
农业科技引用本文复制引用
李文旭,常阳,杨攀,王美芳,何盛莲,吴政卿,雷振生,晁岳恩..郑麦366α-醇溶蛋白基因的克隆及生物信息学分析[J].河南农业科学,2017,46(1):13-18,25,7.基金项目
国家自然科学基金青年基金项目(31501382) （31501382）
河南省农业科学院优秀青年基金项目(2013YQ02) （2013YQ02）
河南省农业科学院自主创新基金项目(2016ZC07) （2016ZC07）
