湖南中医药大学学报2017，Vol.37Issue(4)：365-368,4.DOI:10.3969/j.issn.1674-070X.2017.04.005
凹叶厚朴用于细胞悬浮培养的愈伤组织的制备研究
Study on the Preparation of Callus of Magnolia officinalis Using on Cell Suspension Culture
摘要
Abstract
Objective To study the callus of Magnolia officinalis suitable for cell suspension culture. Methods Callus induction and subculture study were carried out through the selection of basic mediums (B5, MS and 1/2MS), explants (stems, phyllophores and leaves) and concentrations of hormone. Results The best basic medium for callus induction of Magnolia biloba was B5. Phyllophor is the best explant for callus induction of magnolia biloba. The best medium for callus induction of magnolia biloba was B5+2.0 mg/L 2,4-D+1.0 mg/L 6-BA. The best subculture medium for callus of Magnolia officinalis was B5+2.0 mg/L NAA+1.0 mg/L 6-BA, callus growth rate reached 2.87 times. Conclusion The callus of Magnolia officinalis suit-able for cell suspension culture has been preliminary screened, which laying the foundation for cell suspension culture of Magnolia officinalis.关键词
凹叶厚朴/愈伤组织/悬浮培养Key words
Magnolia officinalis/callus/suspension culture分类
医药卫生引用本文复制引用
谢燕燕,卫梅,谢德金,陈剑成,杨德明,荣俊冬,陈礼光,郑郁善..凹叶厚朴用于细胞悬浮培养的愈伤组织的制备研究[J].湖南中医药大学学报,2017,37(4):365-368,4.基金项目
"十二五"国家科技支撑计划项目(2011BAI01B06)资助 （2011BAI01B06）
福建省科技创新平台(2008Y2001)资助. （2008Y2001）
