湖南中医药大学学报2017，Vol.37Issue(4)：369-372,4.DOI:10.3969/j.issn.1674-070X.2017.04.006
葛根素纳米晶的制备工艺及其表征
Characteristics and Process Technology of Peurarin Nanocrystals
摘要
Abstract
Objective In this study, the production, process technology and effects of particle size of puer-arin nanocrystals were investigated, and the puerarin nanocrystals were characterized. Methods High pressure homogenization (HPH) was used for formula and production studies. Particle size as an indicator, we studied the effects of stabilizers, drug/stabilizer ration, drug input and production temperature on the production of peurarin nanocrystals, and then drying method were introduced to study the solidification of nanocrystals. We introduced scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for morphologic observation for crystalline study. Results In the production study, puer-arin nanocrystal with diameter of 20~50 nm could be produced after using PVP K30 as stabilizer, with drug/stabilizer ratio 1:2, temperature above 43℃.The amounts of puerarin input has no significant influence on nanocrystals production. In poilet scaled experiments, nanocrystals with puerarin input of 10% (w/v) were produced at 700 bar for 5 cycles, and the nanocrystals pre-pared were solidified by freezing drying and spray drying. We found that after transferred puerarin into nanocrystals, the irregu-lar shape for puerarin was changed to spherical shape for nanocrystals. Conclusion The formula and production processes esta-bilished were reliable and repeatability, the puerarin nanocrystals with diameter below 100 nm could be prepared effectively.关键词
葛根素/纳米晶/制备工艺/粒径/表征Key words
puerarin/nanocrystals/process technology/particle size/superficial characteristics分类
医药卫生引用本文复制引用
杜俊锋,涂亮星,胡凯莉,奉建芳..葛根素纳米晶的制备工艺及其表征[J].湖南中医药大学学报,2017,37(4):369-372,4.基金项目
国家 "973" 计划项目 (2009CB930300) （2009CB930300）
上海市优秀技术带头人计划项目 (13XD1425300) （13XD1425300）
上海市科委纳米专项项目(11nm0506700). （11nm0506700）
