湖南中医药大学学报2017，Vol.37Issue(4)：402-405,4.DOI:10.3969/j.issn.1674-070X.2017.04.016
电针内关穴预处理对心肌缺血再灌注损伤大鼠线粒体膜电位、腺苷A1受体的影响
Effects of Electroacupuncture at Neiguan Point on Mitochondrion Membrane Potential and Adenosine A1 Receptor in Rats with Myocardial Ischemia-Reperfusion Injury
摘要
Abstract
Objective To explore the cardioprotective mechanisms of electroacupuncture (EA) at Neiguan point and ob-serve the effect of mitochondrial membrane potential and adenosine A1 receptors in rats with myocardial ischemia-reperfusion injury. Methods The 40 SD male rats were randomly divided into the sham-operation group, ischemia reperfusion (mod-el group), EA Neiguan group and EA Huantiao group, 10 rats in each group. The models were established by ligating the coronary artery. The EA Neiguan group and EA Huantiao group were given electroacupuncture stimulation pretreatment 20 min every day lasting for 7 days before establishing MIRI model respectively. The mitochondrial membrane potential was tested by fluorescent technique. The expression of adenosine A1 receptor was determined by Immunohistochemical method. Results The mitochondrial membrane potential and adenosine A1 receptor contents of model group decreased significantly than those of control sham-operation group with obviously statistical difference (P<0.01). The mitochondrial membrane potential of EA Neiguan group increased than those of model group, control sham-operation group and EA Huantiao group (P<0.05). The adenosine A1 receptor increased significantly than model group, EA Huantiao group and control sham-operation group (P<0.01). But there was no significandy difference between model group and EA Huantiao group (P>0.05). Conclusion The EA at Neiguan point preconditioning could induce the expression of adenosine A1 receptor, increase mitochondrial membrane poten-tial, and have a protection on myocardial cells.关键词
心肌缺血再灌注损伤/电针/内关穴/线粒体膜电位/腺苷A1受体Key words
MIRI/eletroacupuncture/Neiguan point/mitochondrial membrane potential/adenosine A1 receptor分类
医药卫生引用本文复制引用
宋瑾,王超,阳仁达,冯果,谭成富,刘薇薇,严洁..电针内关穴预处理对心肌缺血再灌注损伤大鼠线粒体膜电位、腺苷A1受体的影响[J].湖南中医药大学学报,2017,37(4):402-405,4.基金项目
国家自然科学基金项目(81574080) （81574080）
湖南省中医药管理局课题(2015211) （2015211）
湖南省高校创新平台开放基金项目(15k093) （15k093）
2015年针灸推拿学湖南省"十二五"重点学科开放基金资助项目(06). （06）
