华西口腔医学杂志2017，Vol.35Issue(2)：183-186,4.DOI:10.7518/hxkq.2017.02.014
P120-连环蛋白介导钙黏蛋白转换促进口腔鳞状细胞癌细胞迁移和侵袭的实验研究
Cadherin switching induced by P120-catenincan promote the migration and invasion of oral squamous cell cancer cells
摘要
Abstract
Objective The main goal is to investigate the role of P120-catenin (P120ctn) in cadherin switching, as well as migration and invasion, of oral squamous cell cancer (OSCC) cells.Methods The plasmid pGFP-V-RS-P120ctn shRNA was used to transfect TSCCA cells and significantly reduce the expression of P120ctn in these cells. Real-time fluorescent quantita-tive polymerase chain reaction and Western blot were conducted to determine the mRNA and protein expression levels of P120ctn, E-cadherin (E-cad), and N-cadherin (N-cad). By contrast, the Transwell cell invasion and cell migration assay was used to determine the invasion and migration capacities before and after the transfection.Results After the plasmid pGFP-V-RS-P120ctn shRNA was transfected into the TSCCA cells, we found that as the P120ctn expression significantly decreased, E-cad mRNA and protein expression decreased significantly. Moreover, N-cad mRNA and protein expression increased sig-nificantly (P<0.05). Lastly, the cell migration and invasion capacities were augmented significantly (P<0.05).Conclusion In OSCC cells, P120ctn may be involved in cadherin switching and promote metastasis and invasion.关键词
P120-连环蛋白/钙黏蛋白转换/口腔鳞状细胞癌Key words
P120-catenin/cadherin switching/oral squamous cell cancer分类
医药卫生引用本文复制引用
陈钟,张美,徐勇..P120-连环蛋白介导钙黏蛋白转换促进口腔鳞状细胞癌细胞迁移和侵袭的实验研究[J].华西口腔医学杂志,2017,35(2):183-186,4.基金项目
Project Supported by Natural Science Foundation of Xiamen Medical College (2013-02). 厦门医学院自然科学基金(Z2013-02) （2013-02）
