渔业科学进展2017，Vol.38Issue(1)：42-47,6.DOI:10.11758/yykxjz.20160805003
促性腺激素调控半滑舌鳎(Cynoglossus semilaevis)卵母细胞孕酮受体膜组分1的表达特征
HCG Regulation of PGRMC1 Expression in the Oocyte of Half-Smooth Tongue Sole (Cynoglossus semilaevis)
摘要
Abstract
In this study we investigated the characteristics and mechanisms of the expression of progesterone receptor membrane component 1 (PGRMC1) during oocyte maturation of the half-smooth tongue sole (Cynoglossus semilaevis).Expression of PGRMC1 mRNA in the oogenesis of the ovarian development stages Ⅳ and Ⅴ were monitored with qRT-PCR technique.The results showed that the highest level of PGRMC1 mRNA appeared in Phase Ⅴ oocytes of the mature C.semilaevis.In general,the transcription of PGRMC1 peaked in different developmental phases of oocyte.This suggested that PGRMC1 participated in ovarian development and maturation of C.semilaevis.Next we tested whether HCG treatment affected PGRMC 1 expression in different stages of oogenesis using qRT-PCR and western blotting analysis.It was shown that HCG could up-regulate the expression of PGRMC1 mRNA and protein,and that 20 IU/ml HCG was more powerful than 10 IU/ml (P＜0.05).The effect of HCG varied in different phases of oocyte and was the most obvious in Phase Ⅴ,indicating that PGRMC1 played an important role in the mature stage.The results above suggested that PGRMC1 could regulate the maturation of C.semilaevis oocytes.Our findings may shed light on future exploration of the role of PGRMC 1 in the reproductive function of C.semilaevis.关键词
半滑舌鳎/孕酮受体膜组分1 (PGRMC1)基因/卵母细胞/HCG调控/表达分析Key words
Cynoglossus semilaevis/PGMRC1 gene/Oocyte maturation/HCG regulation/Expression analysis分类
农业科技引用本文复制引用
张金勇,柳学周,史宝,徐永江..促性腺激素调控半滑舌鳎(Cynoglossus semilaevis)卵母细胞孕酮受体膜组分1的表达特征[J].渔业科学进展,2017,38(1):42-47,6.基金项目
国家自然科学基金项目(31201982)、国家鲆鲽类产业技术体系(CARS-50)和山东省优秀中青年科学家科研奖励基金项目(BS2013SW042)共同资助[This work was supported by the National Natural Science Foundation of China (31201982),China Agriculture Research System (CARS-50),and Shandong Youth Scientist Awards Foundation (BS2013SW042)] （31201982）
