渔业科学进展2017，Vol.38Issue(1)：73-80,8.DOI:10.11758/yykxjz.20160816003
GH/IGF-Ⅰ轴对半滑舌鳎(Cynoglossus semilaevis)卵巢发育的调控作用
Physiological Role of GH/IGF-Ⅰ Axis in Ovarian Development of Cynoglossus semilaevis
摘要
Abstract
In order to deeply understand underlying mechanisms for regulating reproductive trait of Cynoglossus semilaevis,the physiological role of GH/IGF-Ⅰ axis in ovarian development was explored.The levels of growth hormone (GH) mRNA of pituitary and IGF-Ⅰ mRNA of liver,brain,and ovary at different ovarian development stages were determined by quantitative real-time PCR (qRT-PCR) analysis.Meanwhile,the expression levels of serum GH,IGF-Ⅰ,estradiol (E2) and testosterone (T) were detected by radioimmunoassay (RIA) and ELISA methods,respectively.Furthermore,the correlation between the mRNA and peptides expression levels of GH,IGF-Ⅰ and gonadosomatic index (GSI),and the one between the expression levels of serum E2 and serum T during ovarian development was calculated with statistical methods.The results showed that,the levels of pituitary GH mRNA significantly increased at stage Ⅳ ovary,and peaked at stage Ⅴ ovary,but decreased significantly post spawning (stage Ⅵ).It has significantly positive relationship with the expression levels of GSI,serum GH and serum IGF-Ⅰ during ovarian development.The serum GH levels showed similar trend with pituitary GH mRNA expression levels.Liver IGF-Ⅰ mRNA achieved the minimum levels at stage Ⅳ and stage Ⅴ ovaries,but with a significant increase at stage Ⅵ ovary.It was significantly positive correlated with brain IGF-Ⅰ mRNA levels and serum IGF-Ⅰ levels,but significantly negative correlated with serum E2 and serum T levels during ovarian development.Brain IGF-Ⅰ mRNA levels significantly increased since stage lⅡ ovary and peaked at stage Ⅳ ovary,and showed a significant negative relationship with serum IGF-Ⅰ levels.Ovary IGF-Ⅰ mRNA levels significantly increased at stage Ⅱ ovary and peaked at stage Ⅳ ovary,then significantly declined at stage Ⅴ ovary until stage Ⅵ ovary.It was significantly correlated with serum E2 levels but negatively correlated with serum IGF-Ⅰ levels.Serum IGF-Ⅰ levels showed similar trend with liver IGF-Ⅰ mRNA levels,which significantly decreased at stage Ⅳ ovary,but then significantly ascended to peak at stage Ⅵ ovary.These results revealed that GH and IGF-Ⅰ synergistically or antagonistically play physiological role in ovarian development of C.semilaevis in endocrine or paracrine ways,and their regulation functions are related with synthesis/secretion during ovary development regulation.The present results could provide a new clue for better understanding the possible mechanism for reproductive regulation of C.semilaevis.关键词
半滑舌鳎/生长激素(GH)/类胰岛素生长因子Ⅰ (IGF-Ⅰ)/卵巢发育/调控机制Key words
Cynoglossus semilaevis/Growth hormone/Insulin-like growth factor Ⅰ/Ovarian development/Regulation mechanism分类
农业科技引用本文复制引用
徐永江,柳学周,石莹,王滨,史宝,张凯,蓝功岗..GH/IGF-Ⅰ轴对半滑舌鳎(Cynoglossus semilaevis)卵巢发育的调控作用[J].渔业科学进展,2017,38(1):73-80,8.基金项目
国家鲆鲽类产业技术体系(CARS-50)和国家自然科学基金(31502145 （CARS-50）
31602133)共同资助[This work was supported by China Agriculture Research System (CARS-50) and the National Natural Science Foundation of China (31502145 （CARS-50）
31602133)]. （）
