家畜生态学报2017，Vol.38Issue(3)：14-18,5.
应用PCR-DGGE分析发酵床养殖仔猪消化道菌群多样性及其演替
Analysis on Diversity and Succession of Bacterial Flora in Digestive Tract of Weaned Piglets on Fermentation Bed by PCR-DGGE
摘要
Abstract
PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor the ecological succession and diversity of bacterial flora in each part of digestive tract of weaned piglets raised on fermentation bed and traditional concrete floor at day 21 and day 42.During the whole experimental process,the number of stomach flora species was small,and the smallest was in the ileum which caused by the inhibition of gastric juice;then the flora got the most bacteria species with rapid proliferation in cecum and colon.Compared with concrete floor,fermentation bed provided a stable temperature,humidity,and abundant flora,which was similar to the digestive tract,which could continuously increase the flora diversity of stomach and colon in both ends of the digestive tract,promote the ileum flora into stable cecum flora,the higher proportion of Lactobacillus was kept which could inhibit or retard proliferation of Campylobacter,Shigella,Escherichia to a certain extent.Fermentation bed is an ideal way of piglets farming which is beneficial to building and maintaining normal digestive tract flora for improving the health and productivity of piglets.关键词
PCR-DGGE/发酵床/仔猪/消化道菌群Key words
PCR-DGGE/fermentation bed/piglets/bacterial flora in digestive tract分类
农业科技引用本文复制引用
柳尧波,傅建功,任素芳,郭立辉,张玉玉,刘玉庆..应用PCR-DGGE分析发酵床养殖仔猪消化道菌群多样性及其演替[J].家畜生态学报,2017,38(3):14-18,5.基金项目
国家科技重大专项项目(2013ZX10004217-003) （2013ZX10004217-003）
山东省济南市高校院所自主创新项目((2009)第18号) （(2009）
