家畜生态学报2017,Vol.38Issue(3):25-30,6.
工厂兔、散养兔和野兔盲肠菌群多样性及菌群结构分析
Analysis of Cecal Community Diversity and Structure in Farm Rabbit, Scatter Rabbit and Hare
摘要
Abstract
The aim of this study was to analyze the cecal community diversity and structure in farm rabbits,scatter rabbits and hares.The cecal content were collected from farm rabbits (n=6),scatter rabbits (n=6) and hares (n=6).Total DNA was extracted and the 16S rDNA V4 to V5 of bacteria was sequenced by HiSeq sequencing technology.Results showed that (1) The observed species,the cecal community abundance and community diversity index of farm rabbits were significantly (P<0.05) higher than that of scatter rabbits and hares.(2) The cecal community structure of them was significantly (P<0.05) different with each other.(3) The main bacterial phyla in farm rabbits,scatter rabbits and hares were Firmicutes(farm rabbits were 71.54%,scatter rabbits were 65.37%,hares were 63.25%) and Bacteriodetes(farm rabbits were 23.78%,scatter rabbits were 30.8%,hares were 21.21%).The special bacterial phylum in hares was Spirochaetae(12.36%).The main bacterial family in farm rabbits,scatter rabbits and hares were Ruminococcaceae(farm rabbits were 39.26%,scatter rabbits were 33.33%,hares were 29.16%),Lachnospiraceae (farm rabbits were 23.04%,scatter rabbits were 28.47%,hares were 21.54%).The special dominant bacterial family of scatter rabbits was Rikenellaceae(19.75%) and the special dominant bacterial family of hares was Spirochaetaceae(12.36 %).In the genus level,the highest bacterial in farm rabbits,scatter rabbits and hares were Bacteroides (9.45 %),Rikenellaceae-RC9-gutgroup (17.68%) and Sphaerochaeta (12.36%),respectively.There was a significant (P<0.05) difference in the cecal community structure of farm rabbits,scatter rabbits and hares while their dominant bacterial phylum all included Firmicutes and Bacteriodetes,and the cecal community diversity of farm rabbits was significantly higher than that of scatter rabbits and hares.关键词
肉兔/野兔/盲肠/菌群多样性/菌群结构Key words
meat rabbit/hare/cecum/community diversity/community structure分类
农业科技引用本文复制引用
宋冰,任战军,宫瑞光,孟繁臣..工厂兔、散养兔和野兔盲肠菌群多样性及菌群结构分析[J].家畜生态学报,2017,38(3):25-30,6.基金项目
家兔肉用性状功能基因解析(K3310216062) (K3310216062)
伊高乐兔工厂化繁育技术(K3360216012) (K3360216012)