基础医学与临床2017，Vol.37Issue(4)：500-505,6.
肾脏纤维化大鼠模型尿液中差异表达基因筛选及生物信息学分析
Differentially expressed gene screening of urinesamples from rats renal fibrosis model and bioinformatics analysis
摘要
Abstract
Objective To investigate the differentially expressed genes of urine samples from renal fibrosis model and carry out bioinformatics analysis.Methods Rat renal fibrosis model was constructed with the method of unilateral ureteric obstruction.Urine were collected from the rats with unilateral obstructive nephropathy and sham group, respectively.Total RNA was extracted and sequencing library was established.Differential expression mRNA were analyzed by GO and KEGG pathway.Known pre-miRNA were detected and novel lncRNA family were classified.Results Compared with the sham group urine, 813 up-regulated mRNA/lncRNA and 213 down-regulated mRNA/lncRNA were collected from the urine of renal fibrosis rats.Conclusions There are significant differential expression profile in urine samples between renal fibrosis rat group and the shame group.With high-throughput transcriptome sequencing and bioinformatics analysis, the exciting possibility was raised for better understanding renal pathologies and development of new diagnostic biomarkers.关键词
肾脏纤维化/生物信息学分析/转录组测序/单侧输尿管结扎Key words
renal fibrosis/bioinformatics analysis/transcriptome sequencing/unilateral ureteric obstruction分类
医药卫生引用本文复制引用
张尚,石变华,孙佳增,刘彦信,郑德先,史娟..肾脏纤维化大鼠模型尿液中差异表达基因筛选及生物信息学分析[J].基础医学与临床,2017,37(4):500-505,6.基金项目
国家重点基础研究发展计划(2013CB530800) （2013CB530800）
