极地研究2017，Vol.29Issue(1)：82-89,8.DOI:10.13679/j.jdyj.2017.1.082
南极菲尔德斯半岛土壤氮循环微生物类群数量与功能活性的初步研究
A PRELIMINARY STUDY ON THE POPULATION AMOUNT AND FUNCTIONAL ACTIVITY OF SOIL NITROGEN CYCLE MICROBES IN FILDES PENINSULA OF ANTARCTICA
摘要
Abstract
Fildes Peninsula is located at the southern end of King George Island in Antarctica. It is an ideal place to study Antarctic ecology and biological resources because of its unique geographical location and ecolog-ical environment. In this study, three different soil sample types were collected from the peninsula. The population sizes of soil nitrogen cycle microbes and their functional activities were determined using the Most Probable Number (MPN) and conventional Griess and Nessler's active color test methods. The expe-rimental results were also comprehensively analyzed regarding the ecology and physicochemical properties of the soil. The objectives were to develop a preliminary understanding of the soil nitrogen cycle microbes of Fildes Peninsula, and to prepare a scientific foundation for further study within this area. The results showed that the population sizes of nitrogen cycle microbes in the 13 collected soil samples in descending order were ammonification bacteria > denitrifying bacteria > nitrobacteria or nitrogen-fixing bacteria. Ex-cept for a few samples, most exhibited differing degrees of nitrification, denitrification, and ammonification activity. Furthermore, all soil samples had relatively high levels of ammonification bacteria and ammonifi-cation activity. The population sizes of denitrifying bacteria and the denitrifying activity of hillslope soils were higher than soils occupied by animals and plants. Although lower levels of nitrobacteria existed in soils occupied by animals and plants, they showed higher nitrifying activity, which might indicate the existence of strains with efficient function. Furthermore, the population sizes of nitrobacteria and the nitrifying activity of hillslope soils varied. This study provides a preliminary reference for further research of the characteris-tics of nitrogen cycling microbes in Antarctica.关键词
菲尔德斯半岛/南极/土壤氮循环微生物/MPN法/菌群数量/功能活性/土壤理化性质Key words
Fildes Peninsula/Antarctica/soil nitrogen cycle microbes/MPN method/population size/functional active intensity/soil physicochemical properties引用本文复制引用
刘杰,张丹丹,董龙龙,王能飞..南极菲尔德斯半岛土壤氮循环微生物类群数量与功能活性的初步研究[J].极地研究,2017,29(1):82-89,8.基金项目
南北极环境综合考察与评估专项(CHINARE2015-02-01)、中央级公益性科研院所基本科研业务专项资金(2015T04)和山东省重点研发计划(2015GSF115004)资助 （CHINARE2015-02-01）
