解放军医学杂志2017，Vol.42Issue(3)：186-189,4.DOI:10.11855/j.issn.0577-7402.2017.03.02
河蚌多糖抗HBV活性及连分式插值法检测
Activity of anodonta polysaccharides against HBV and its detection by continued fraction interpolation method
摘要
Abstract
Objective To explore the anti-HBV activity of anodonta polysaccharides (AP) and dose-effect relationship in vitro.Methods HepG2.2.15 cells were cultured in vitro and incubated at 37℃ for nine days with AP at a dilution ratio of 1∶10.The expression of HBsAg and HBeAg were detected using ELISA and HBV-DNA copies were detected by real-time fluorescent quantitative PCR.Based on Thiele-type continued-fraction interpolation method,the anti-HBV activity of AP was studied,and the IC50 and the maximum inhibition rate were calculated.Results AP had significant inhibitory effect on the expression of HBsAg and HBeAg in HepG2.2.15 cells in vitro,as well as HBV DNA replication.By Thiele-type continued-fraction interpolation the equations of dose-effect relationship were obtained to determine the maximum inhibition rates of AP on HBeAg and HBsAg secretion being 47.7％ and 56.4％,and the IC50 inhibiting the expression of HBeAg being 143.7mg/L.AP was also able to inhibit HBV-DNA replication and the maximum inhibition rate was 17.8％ with the same method above.Conclusion Anodonta polysaccharides have anti-HBV activity.The thiele-type continued-fraction interpolation method is simple and practical and could be used as a new method for the analysis of drug activity.关键词
河蚌多糖/肝炎病毒,乙型/连分式插值Key words
anodonta polysaccharides/hepatitis B virus/continued-fraction interpolation分类
医药卫生引用本文复制引用
朱涛,赵前进,许礼发,李朝品..河蚌多糖抗HBV活性及连分式插值法检测[J].解放军医学杂志,2017,42(3):186-189,4.基金项目
国家自然科学基金(60973050) （60973050）
安徽省教育厅高校省级自然科学重点研究项目(KJ2008A014)This work was supported by the National Natural Science Foundation of China(60973050),and Key Project of Provincial Natural Science of Educational Commission of Anhui Province (KJ2008A014) （KJ2008A014）
