解放军医学杂志2017，Vol.42Issue(3)：196-201,6.DOI:10.11855/j.issn.0577-7402.2017.03.04
面神经离断伤后核团微环境中NG2阳性细胞及各类胶质细胞的反应
Response of NG2 positive cells and other glial cells in the microenvironment of facial nucleus after facial nerve axotomy
摘要
Abstract
Objective To study the response of NG2 positive and other glial cells in the facial nucleus after facial nerve axotomy,and explore the changes of the microenvironment in the facial nucleus.Methods Rat facial nerve axotomy models were established.Immunofluorescence double staining,and immunohistochemical staining combined with cresyl violet staining were used to observe the response of NG2 cells and other glial cells,and Western blotting was performed to test NG2 protein expression in facial nucleus at postoperative 1,2,7,14,and 28 days.Results Microglia formed dense circles closely around the injured neurons.Astrocytes formed wreath-like structure near the injured neurons.NG2 protein in the injured nucleus has a regular timephase change and NG2 positive cells showed an extensive detachment of synaptic terminals on the damaged neurons after facial nerve axotomy.NG2 cell response was almost the same as microglia.Conclusions All kinds of glial cells may be involved in the formation of glial scar.NG2 positive cells could insulate the damaged neurons against the potential damage from the excitatory input.关键词
面神经损伤/面神经核/NG2阳性细胞/神经胶质Key words
facial nerve injuries/facial nucleus/NG2 positive cells/neuroglia分类
医药卫生引用本文复制引用
朱鴷,结祥,刘安堂,汪汇,张文俊,江华..面神经离断伤后核团微环境中NG2阳性细胞及各类胶质细胞的反应[J].解放军医学杂志,2017,42(3):196-201,6.基金项目
国家自然科学基金青年项目(81301054) （81301054）
上海市自然科学基金青年项目(13ZR1450600) （13ZR1450600）
上海市卫生局青年科研项目(2012Y142)This work was supported by the Youth Project of National Natural Science Foundation of China (81301054),the Youth Project of Natural Science Foundation of Shanghai (13ZR1450600) and the Youth Research Project of Shanghai Municipal Bureau of Health (2012Y142) （2012Y142）
